Biochemical Assessment of Picralima nitida Seeds on Oxidative Stress Parameters of Albino Rats

Abstract

The effects of different percentage concentrations (5%, 10%, 20% and 30%) of ground seeds of Picralima nitida on the concentrations of ascorbic acid (AA), glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT) and lactate dehydrogenase (LDH) of albino rats after 28 days were investigated. Albino rats fed without ground seeds of Picralima nitida served as the control. The concentrations of AA, GSH and the activities of SOD, CAT and LDH from groups of albino rats that were fed with different concentrations of ground seeds of Picralima nitida were determined/assayed using standard methods. Results showed that there were no significant (p<0.05) difference in the concentrations of glutathione of rats fed with 5% feed-formulated with ground seeds of Picralima nitida when compared to those of the control. However, there were significant (p<0.05) differences in the concentrations of AA and activities of SOD, CAT and LDH in the rats fed with 5%, 10%, 20% and 30% when compared to those of the control. Also, there was a significant (p<0.05) reduction observed in the glutathione concentration of rats fed with10%, 20% and 30% feed formulated with ground seeds Picralima nitida. The values obtained showed that, as the percentage concentration of ground seeds Picralima nitida in the formulated feed increased, the concentrations of ascorbic acid and glutathione decreased in contrast to the activities of SOD, CAT and LDH indicating that these changes are concentration-dependent. The high concentrations of the ground seeds of Picralima nitida in the formulated feed might have caused the observed changes in these oxidative stress parameters in the serum of experimental albino rats.