Bioassay of Phytochemicals Isolated from Chloroform Extracts of Azadirachta indica Leaves

Abstract

The present study investigated the chemical constituents isolated from Azadirachta indica chloroform extract. The extraction of leaves was done using Soxhlet extraction apparatus. To isolate and identify the antibacterial fraction from Azadirachta indica chloroform extract, TLC-bioautography was carried out. Phenol 3,5-bis (1,1-dimethyl ethyl), Phthalic acid bis (7-methyloclyl), Dido decyl phthalates, Oxalic acid, allyl hexadecyl ester, and 2-Piperidinone, N-(4-bromo-n-butyl) were the five primary antibacterial chemicals identified by the GC-MS study. The findings of the FTIR study revealed the presence of functional groups C-H str, C=O str, and C=C str as well as alcohol and the carboxylate ion. While the 13C NMR data demonstrated the existence of carbonyl, aromatic carbon, quaternary carbon, olefinic carbon, and methyl group, and the 1H NMR results revealed the presence of aliphatic OH, methyl, aromatic OH, and olefinic proton. The phytoconstituents detected using GC-MS analysis showed a wide range of pharmacological properties, including antioxidant, antibacterial, antifungal, anti-inflammatory, and antimalarial effects. Thus, Azadirachta indica plant has a high concentration of medicinal phytoconstituents that can be used to make antimicrobial medications to fight against plant pathogenic bacteria.