Cell Viability Study of ZnCuInS/ZnS–TPPS4 Conjugates against Different Cell Lines as a Promising Fluorescent Probe
Ncediwe Tsolekile, Sundararajan Parani, Thabang Calvin Lebepe, Rodney Maluleke, Vuyelwa Ncapayi, Mangaka Clara Matoetoe, Sandile Phinda Songca, Oluwatobi Samuel Oluwafemi
Organics · pp. 126–136 · Published 17 Mar 2023
10.3390/org4010010Abstract
In the present work, we report a simple synthetic strategy for fabricating ZnCuInS/ZnS–TPPS4 conjugates and study its cytotoxicity as a promising material for imaging and phototherapy applications. The quaternary QDs were synthesized using eco-friendly materials such as glutathione and water as a solvent, while the anionic 10,15,20-(4-sulphonatophenyl) porphyrin (TPPS4) was synthesized via the acidification of a meso-tetraphenylporphyrin precursor. Interest in TPPS4 results from its high-water dispersity, stability, and ability to generate singlet oxygen. Conjugation of ZnCuInS/ZnS QDs with TPPS4 was performed by titrating porphyrin with different amounts of ZnCuInS/ZnS QDs while keeping all other experimental parameters constant. Comparative analysis of the conjugate to the bare QDs and porphyrin revealed enhanced spectral and photophysical properties. Comparative cytotoxicity assays were performed for TPPS4 and ZnCuInS/ZnS–TPPS4 conjugates in BHK21, Hela, A549, Hek 293 and B16-F10 Nex 2 cell lines using the MTT cell viability assay. The results showed negligible in vitro cytotoxicity indicating the conjugate is an excellent and biocompatible candidate for imaging and phototherapy applications.
References (26)
- 1 Tsolekile, 2017, Evolution of ternary I–III–VI QDs: Synthesis, characterization, and application [DOI]
- 2 Jia, 2017, Tunable emission properties of core-shell ZnCuInS-ZnS quantum dots with enhanced fluorescence intensity [DOI]
- 3 Ilaiyaraja, 2016, Synthesis of Cu-Deficient and Zn-Graded Cu-In-Zn-S Quantum Dots and Hybrid Inorganic-Organic Nanophosphor Composite for White Light Emission [DOI]
- 4 Yang, 2019, Highly luminescent Zn–Cu–In–S/ZnS core/gradient shell quantum dots prepared from indium sulfide by cation exchange for cell labeling and polymer composites [DOI]
- 5 Nady, 2019, Room temperature synthesis of aqueous ZnCuInS/ZnS quantum dots [DOI]
- 6 Zhang, 2015, The Composition Effect on the Optical Properties of Aqueous Synthesized Cu–in–S and Zn–Cu–in–S Quantum Dot Nanocrystals [DOI]
- 7 Fang, 2023, Quaternary alloyed quantum dots with a wide-ranging tunable emission for high color-rendering white light-emitting diodes [DOI]
- 8 Abdulaeva, 2020, Heterocycle-appended porphyrins: Synthesis and challenges [DOI]
- 9 Moreira, 2020, An insight into the synthesis of cationic porphyrin-imidazole derivatives and their photodynamic inactivation efficiency against Escherichia coli [DOI]
- 10 Castriciano, 2017, Poly(carboxylic acid)-Cyclodextrin/Anionic Porphyrin Finished Fabrics as Photosensitizer Releasers for Antimicrobial Photodynamic Therapy [DOI]
- 11 Yang, 2020, Biocompatible conjugated porphyrin nanoparticles with photodynamic/photothermal performances in cancer therapy [DOI]
- 12 Bera, 2018, Porphyrin-Gold Nanomaterial for Efficient Drug Delivery to Cancerous Cells [DOI]
- 13 Kyropoulou, 2020, Porphyrin Containing Polymersomes with Enhanced ROS Generation Efficiency: In Vitro Evaluation [DOI]
- 14 Rojkiewicz, 2013, The synthesis of new potential photosensitizers [DOI]
- 15 Chen, 2018, Design of a novel naked-eye and turn-on fluorescence sensor based on the 5,10,15,20-(4-sulphonatophenyl) porphyrin (TPPS4)-Hg2+ system: Monitoring of glutathione (GSH) in real samples and DFT calculation [DOI]
- 16 Parra, 2018, Stimulation of Cysteine-Coated CdSe/ZnS Quantum Dot Luminescence by meso-Tetrakis (p-sulfonato-phenyl) Porphyrin [DOI]
- 17 Kou, 2017, Porphyrin photosensitizers in photodynamic therapy and its applications [DOI]
- 18 Managa, 2019, Photophysical properties and photodynamic therapy activity of a meso-tetra(4-carboxyphenyl)porphyrin tetramethyl ester-graphene quantum dot conjugate [DOI]
- 19 Ibarra, 2020, Photodynamic inactivation of ESKAPE group bacterial pathogens in planktonic and biofilm cultures using metallated porphyrin-doped conjugated polymer nanoparticles [DOI]
- 20 Tsolekile, 2020, Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS-Meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells [DOI]
- 21 Yue, 2018, Comparative advantages of Zn-Cu-In-S alloy QDs in the construction of quantum dot-sensitized solar cells [DOI]
- 22 Guo, 2013, Synthesis of Zn-Cu-In-S/ZnS Core/Shell Quantum Dots with Inhibited Blue-Shift Photoluminescence and Applications for Tumor Targeted Bioimaging [DOI]
- 23 Lin, 2020, High color rendering index of ZCIS quantum dots-based white light-emitting diodes
- 24 Jia, 2016, RSC Advances A facile method for the synthesis of CuInS2–ZnS quantum dots with tunable photoluminescent [DOI]
- 25 Park, 2011, CuInS2/ZnS core/shell quantum dots by cation exchange and their blue-shifted photoluminescence [DOI]
- 26 Jawhar, 2020, Preparation of highly emissive and reproducible Cu–In–S/ZnS core/shell quantum dots with a mid-gap emission character [DOI]
Cited by 10
Kui Wang, Hui-Na Ma, Jia-Xuan Song · Food Chemistry · 2024
Nande Mgedle, Oluwatobi Samuel Oluwafemi · Journal of Inorganic and Organometallic Polymers and Materials · 2025
Nande Mgedle, Oluwatobi Samuel Oluwafemi · Reactions · 2024
Bhavtosh Kikani · Elsevier eBooks · 2026
Nande Mgedle, Oluwatobi Samuel Oluwafemi · Next Materials · 2026
Cátia Vieira, Maria Bartolomeu, C. Monteiro · Aquaculture · 2024
Showing 6 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Boranils: Versatile Multifunctional Organic Fluorophores for Innovative Applications — shares topic coverage
- Specific Bifunctionalization on the Surface of Phosphorus Dendrimers Syntheses and Properties — shares topic coverage
- Thienothiophene Scaffolds as Building Blocks for (Opto)Electronics — shares topic coverage
- Looking for a Safe Bridge: Synthesis of P3HT-Bridge-TBO Block-Copolymers and Their Performance in Perovskite Solar Cells — shares topic coverage
- Triple Benefits of Cardanol as Chain Stopper, Flame Retardant and Reactive Diluent for Greener Alkyd Coating — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
10
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.