Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage
Lincon Rafael da Silva, Marina Gabriela Marques, Paulo Henrique Pereira Costa Muniz, Thiago Alves Santos de Oliveira, Elizabeth Amélia Alves Duarte, Fabricio Rodrigues, Solange Xavier dos Santos, Daniel Diego Costa Carvalho
Journal of Scientific Research and Reports · pp. 68–77 · Published 3 Mar 2021
10.9734/jsrr/2021/v27i130350Abstract
After long periods of storage, plant pathogen isolates lose their sporulation capacity. The objective of this study was to evaluate re-isolation methodologies for recovering sporulation of Pestalotiopsis grandis-urophylla isolates after subjection to a long period of storage. Isolates of P. grandis-urophylla were kept for 14 months on Petri dishes with PDA medium at 10°C. After this period, the isolate colonies showed reduced mycelial growth and no sporulation. The isolates were inoculated on healthy Eucalyptus grandis-urophylla leaves, and after ten days they were subjected to three re‑isolation methods: scraping of the lesions (S) removing of injured plant tissue fragments, followed by disinfestation (D) and without disinfestation (WD). Then, the purified isolates were evaluated for the recovery of its sporulation ability. The different methods for re-isolation resulted in the occurrence of differences among the isolates, showing that sporulation is an isolate-dependent feature. The three methods (S, WD and D) allowed the sporulation recovery of P. grandis-urophylla, even after these isolates have been subjected to 14 months.
Cited by 0
No indexed citations yet.
Related research
- Prevalence of Fusarium Species Associated with Peach Decline in Tunisian Nurseries — shares topic coverage
- Characterization and Identification of Bacteria Present in the Bath Towels of Female Students in Rivers State University Hostels — shares topic coverage
- Peste Des Petits Ruminants: An Update — shares topic coverage
- Cross-Pathogenicity of Colletotrichum sp. Isolates from Avocado and Banana Fruits in Côte d’Ivoire — shares topic coverage
- Cultural, Morphological, Pathogenic and Molecular Characterization of Isolates of Fusarium spp. — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.