Association of Glu298Asp Polymorphism of eNOS Gene with Cardiovascular Diseases
Aldijana Avdić, Admir Kurtcehajic, Vesna Hadziavdic, Amela Hercegovac, Aldijana Dautbasic, Senad Brzovic, Suad Siranovic, Maja Podjanin, Jasmina Dedić, Suada Cipurkovic, Irma Huskic
Biotechnology Journal International · pp. 29–37 · Published 10 Oct 2022
10.9734/bji/2022/v26i4654Abstract
Molecular-genetic diagnostics of polygenic diseases is a new and interesting area in laboratory diagnostics, especially in the area of cardiovascular diseases, as one of the leading causes of mortality in the world population. Aims: The aim of the paper was to analyse variants of the endothelial nitric oxide synthase gene (NOS3) (Glu298Asp/G894T) in the human population of Tuzla Canton in relation to cardiovascular diseases. Study Design: The study included 112 respondents of both sexes over 18 years old. The experimental group for the analysis of the polymorphism (Glu298Asp) of the endothelial nitric oxide synthase gene included 56 respondents of both sexes with cardiovascular disease (hypertension), while the control group comprised 56 healthy respondents of both sexes without a prior history of cardiovascular disease (sample/control). Place and Duration of Study: Blood sampling was performed at Medical Center "Plava Poliklinika", “Plava Medical Group”, Department of Biochemistry, Microbiology and Genetics, Tuzla. DNA isolation and molecular-genetic analysis of the samples were performed in Laboratory for scientific research at the Department of Biology, Faculty of Natural Sciences and Mathematics in Tuzla. Methodology: The genotyping of eNOS Glu298Asp polymorphism for all respondents was determined by an optimized method based on PCR-RFLP reaction. Results: In the total sample of respondents, the highest genotype frequencies of the eNOS gene were recorded for the GG genotype (53.5%) and the GT genotype (35.7%). The lowest frequency was recorded for the TT genotype, which was 10.8%. Conclusion: The results obtained in the study provide good guidelines for further study of a molecular-genetic association between a high number of gene candidates and cardiovascular diseases, which will contribute to the incorporation of these results into the existing regional and European genetic database.
References (10)
- 1 Association of endothelial nitric oxide synthase gene polymorphisms with classical risk factors in development of premature coronary artery disease [DOI]
- 2 The polymorphism G894 T of endothelial nitric oxide synthase (eNOS) gene is associated with susceptibility to essential hypertension (EH) in Morocco [DOI]
- 3 Association of eNOS Glu298Asp gene polymorphism with essential hypertension in Asian Indians [DOI]
- 4 Endothelial Nitric Oxide Synthase Gene Polymorphisms and the Risk of Hypertension in an Indian Population [DOI]
- 5 Association between endothelial nitric oxide synthase gene polymorphisms and risk of ischemic stroke: A meta-analysis [DOI]
- 6 Pharmacogenetic Implications of eNOS Polymorphisms (Glu298Asp, T786C, 4b/4a) in Cardiovascular Drug Therapy [DOI]
- 7 Coronary Artery Ectasia Is Associated with the c.894G>T (Glu298Asp) Polymorphism of the Endothelial Nitric Oxide Synthase Gene [DOI]
- 8 Association of endothelial nitric oxide synthase Glu298Asp gene polymorphism in psoriasis cases with hypertension [DOI]
- 9 Lack of association between eNOS Glu298Asp gene polymorphism and carotid atherosclerosis in a Serbian population [DOI]
- 10 Identification of genetic risk factors associated with ischaemic stroke in young Mexican patients [DOI]
Cited by 1
Edin Kurtović, A. Avdić, Melisa Šehić · Genetics & Applications · 2026
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