Skip to content
Research Article Open access CC BY 4.0

Assessment of Effects of Amended Bioremediation of Toxic Metal-polluted Soil using Organic Composites and Bacillus sp on Plant Growth Parameters

Maximus Chibuoyi Korie, Ferdinand Ozurumba Uzoma Osuala, Jude-Anthony Nnama Ogbulie, Josephat Nwabueze Okereke, Sylvia Onyinyechi Anyadoh-Nwadike, Colman Chikwem Ibe, Moses Ekene Nwachukwu

Biotechnology Journal International · pp. 21–38 · Published 7 May 2024

10.9734/bji/2024/v28i3721

Abstract

It has become pertinent to develop improved bioremediation techniques, to avert the deleterious effects of heavy metal pollution on agricultural productivity, and safety of its produce. In this study, cow dung, poultry wastes and their composites used as organic amendments were collected and processed, while Bacillus sp used as plant growth promoting rhizosphere was isolated and identified. Then 24 kg of fertile soil was polluted by introducing 20 mL of 0.1M lead acetate solution, before bagging 5 kg of polluted soil into ten perforated nylons. Soil sample was then analyzed before and after treatment. After mixing with Bacillus sp suspension, two of the pot experiments were amended with each of 0.5 kg cow dung only, 0.5 kg poultry wastes only and 1:1 ratio of their composites. Control samples were set up without amendment, and they were bioremediated for 25 days. Following germination of sown corn seedlings, heights of their shoots and number of leaves were monitored on day 4, weeks 2, 4 and 7, before harvesting for analysis together with soil samples. Results obtained revealed that seedlings grown on treated samples as well as properties of treatment of soil were generally better than those of control samples. Highest percentage increase in organic matter content of soil was recorded in treatment with mixture of cow dung only and Bacillus sp, with 9.28% and 7.63% increase at 33.75 mg/kg and 52.55 mg/kg of lead (Pb) pollution. Seedlings grown on samples treated with poultry wastes only, recorded the highest protein content of 161.25±5.5 mg/g, 104.99±4.95 mg/g and 79.75±3.2 mg/g protein contents, at 32.75 mg/kg, 42.02 mg/kg and 52.44 mg/kg levels of Pb pollution, respectively. The amendments also reduced bioaccumulation of Pb in corn seedlings, which was in the order; stem<leaf<root. Therefore, these amendments can be exploited for improved bioremediation of heavy metals.

Pollution bioremediation phytotoxicity plant growth chlorophyll content

References (27)

  1. 1 Remediation techniques for heavy metal-contaminated soils: Principles and applicability [DOI]
  2. 2 Toxic Heavy Metal and Metalloid Accumulation in Crop Plants and Foods [DOI]
  3. 3 Role of organic amendments on enhanced bioremediation of heavy metal(loid) contaminated soils [DOI]
  4. 4 Effect of bamboo and rice straw biochars on the mobility and redistribution of heavy metals (Cd, Cu, Pb and Zn) in contaminated soil [DOI]
  5. 5 A review on heavy metal contamination in the soil worldwide: Situation, impact and remediation techniques
  6. 6 Heavy Metal Contamination of Soils: Sources, Indicators and Assessment
  7. 7 Conocarpus biochar as a soil amendment for reducing heavy metal availability and uptake by maize plants [DOI]
  8. 8 Past, present and future trends in the remediation of heavy-metal contaminated soil - Remediation techniques applied in real soil-contamination events [DOI]
  9. 9 Heavy metals immobilization and improvement in maize (Zea mays L.) growth amended with biochar and compost [DOI]
  10. 10 Impact of organic amendments (biochar, compost and peat) on Cd and Zn mobility and solubility in contaminated soil of the Campine region after three years [DOI]
  11. 11 Immobilization of heavy metals in contaminated soils by modified hydrochar: Efficiency, risk assessment and potential mechanisms [DOI]
  12. 12 Harmful Impacts of Heavy Metal Contamination in the Soil and Crops Grown Around Dumpsites [DOI]
  13. 13 Effects of Organic Amendments on Soil Fertility and Environmental Quality: A Review [DOI]
  14. 14 Quantification of total protein content from some traditionally used edible plant leaves: A comparative study [DOI]
  15. 15 Soil Enzyme Activity Response under the Amendment of Different Types of Biochar [DOI]
  16. 16 Effect of Manure and Compost on the Phytostabilization Potential of Heavy Metals by the Halophytic Plant Wavy-Leaved Saltbush [DOI]
  17. 17 Root Characteristics and Metal Uptake of Maize (Zea mays L.) under Extreme Soil Contamination [DOI]
  18. 18 Ingestion and inhalation of metal(loid)s through preschool gardening: An exposure and risk assessment in legacy mining communities [DOI]
  19. 19 The impact of heavy metal contamination on soil health [DOI]
  20. 20 Nitrogen fertilizer enhances zinc and cadmium uptake by hyperaccumulator Sedum alfredii Hance [DOI]
  21. 21 Improving quality of metal-contaminated soils by some halophyte and non-halophyte forage plants [DOI]
  22. 22 Heavy metal accumulation in leafy vegetables grown in industrial areas under varying levels of pollution [DOI]
  23. 23 Isolation and Identification of Bacillus Species from Soil for Phosphate, Potassium Solubilisation and Amylase Production [DOI]
  24. 24 Phytoremediation potential of two maize varieties cultivated on metal-particulate-contaminated soil [DOI]
  25. 25 Remediation of soil polluted with spent oil using cow dung [DOI]
  26. 26 Remediation of Heavy Metals Contaminated Soils: A bibliometric Network Analysis [DOI]
  27. 27 Effect of organic amendments on soil fertility and quality of organic zucchini under greenhouse condition [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

19

Page views

0

PDF downloads

2

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.