Assessment of 8-Hydroxy 2-Deoxyguanosine as Salivary Biomarker for Periodontitis Patients with or without Diabetes Mellitus

Abstract

Aim: The aim of this study is to assess the levels of 8-hydroxy-2 deoxyguanosine as a salivary biomarker for periodontitis with or without diabetes mellitus. Study Design: Cross-sectional study Materials and Methods: 30 patients were included in the study and were categorised into three groups: periodontal health (Group a), patients with periodontitis without diabetes mellitus (Group b) and patients with periodontitis only (Group c). Unstimulated salivary samples were taken. The OHdG concentration was evaluated using Sandwich-enzyme linked immunosorbent assay by commercially available human OHdG 96 well ELISA kit. The data were statistically analysed by One-Way -ANOVA. The Newman-Keuls multiple comparison test was used to test the significance at the levels of p<0.05. Results: Salivary OHdG concentration in periodontal health, periodontitis with diabetes mellitus (P+DM), Periodontitis only (P) were analysed.  Salivary OHdG concentration was found to be significantly higher (p<0.05) in periodontitis with diabetes mellitus (38±2.82 ng/L) when compared with periodontitis only (20.5±2.12 ng/L) and also when compared with healthy controls (11.5±2.12 ng/L). Conclusion: Diabetic patients with periodontitis have increased 8 hydroxy 2 deoxyguanosine than non-diabetic counterparts. Thus salivary 8 hydroxy 2 deoxyguanosine is an effective non-invasive biomarker to assess periodontitis among patients.