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Research Article Open access CC BY 3.0

Arachis hypogaea L. Direct Organogenesis and SDS - PAGE Analysis in Groundnut

S. Palanivel, S. Parvathi, V. Muniappan, C. Deepa, A. Abuthahir

Biotechnology Journal International · pp. 720–732 · Published 18 Jun 2014

10.9734/BBJ/2014/9237

Abstract

The present research work was carried out to observe the effect of various concentrations of Benzyl amino purine (BAP) in terms of multiple shoot induction with special emphasis on qualitative and quantitative changes of proteins during multiple shoot formation. From whole embryonated cotyledons of groundnut. The shoot induction medium containing different concentrations of Benzyle amino purine ranged from 5.0 to 25.0mg/l along with lower concentration of Indole acetic acid (IAA).The multiple shoots were observed in all the BAP concentrations at a varying levels. Among the various levels of BAP, 25.0mg/l of BAP plus 0.50mg/l of IAA was found to be the most effective in terms of multiple shoot induction. The growth parameters like plantlet height, fresh and dry weight also highly influenced by the concentration of the BAP. The root induction was achieved in micropropagated shoots by using Indole butyric acid (IBA) at a concentrations of 1.0 to 5.0mg/l. The mean number of roots, root length was highly influenced by IBA. In all the concentrations of IBA the rooting was associated with moderate and heavily associated basal callusing. The quantitative estimation of protein content in regenerated shoots also increased with higher concentrations of BAP. The SDS-PAGE analysis showed that there was a nine visible bands with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were observed in multiple shoots derived from all the concentrations of BAP. Whereas in whole embryonated cotyledonary explants there was a entirely different protein banding pattern with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were recorded. 

Arachis hypogaea multiple shoot induction quantitative and qualitative analysis of proteins rooting

References (14)

  1. 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
  2. 2 In Vitro Culture of Higher Plants [DOI]
  3. 3 Recent developments in transgenics for abiotic stress in legumes of the semi-arid tropics
  4. 4 Plantlet regeneration from different callus cultures of pigeonpea (Cajanus cajan L.) [DOI]
  5. 5 Cytokinin stimulation of abscission in lemon pistil explants [DOI]
  6. 6 The probable genome donors to Arachis hypogaea L. based on arachin seed storage protein [DOI]
  7. 7 In vitro micropropagation of peanut (Arachis hypogaea) through direct somatic embryogenesis and callus culture.
  8. 8 Response of Groundnut Varieties to Plant Growth Regulator (BAP) to Induce Direct Organogenesis
  9. 9 In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants [DOI]
  10. 10 Direct somatic embryogenesis from axes of mature peanut embryos [DOI]
  11. 11 Callus induction and plantlet regeneration from mature cotyledonary segments of groundnut (Arachis hypogaea L.) [DOI]
  12. 12 Multiple Shoot Formation and Efficient Root Induction in Groundnut (Arachis hypogaea L.)
  13. 13 Changes in protein composition and protein phosphorylation during somatic embryogenesis and plant regeneration in peanut (Arachis hypogaea L.) [DOI]
  14. 14 Partial morphogenesis in peanut (Arachis hypogaea L.) callus cultures.

Cited by 1

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