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Research Article Open access CC BY 4.0

Applications of CRISPR/Cas9 for Biotic and Abiotic Stress Resistance of Rice (Oryza sativa)

Kunal Dhadge, Puneet Walia

Journal of Advances in Biology & Biotechnology · pp. 172–179 · Published 6 Jun 2024

10.9734/jabb/2024/v27i7977

Abstract

Biotechnology has played an important role in the development of varieties with enhanced biotic and abiotic stress tolerance, higher yield potential and an improved resistance towards major diseases and pathogens. The precision of the CRISPR-Cas9 system's target recognition is notably dependable because it employs the Watson and Crick model to identify target sites and utilizes sequence analysis to pinpoint potential off-target locations.  In a study carried out to develop resistance against bacterial blight on CO51 variety of rice, a single guide RNA was designed using the structure and sequence information obtained through Ensembl Plants database.  The integration of CRISPR/Cas9 technology with traditional breeding methods and other advanced biotechnological tools may further enhance the development of stress-resilient rice varieties with improved productivity and sustainability.

Stress-resilient rice varieties abiotic factors biotechnology CRISPR-Cas9

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Cited by 10

Unleashing the Potential of CRISPR/Cas9 Genome Editing for Yield-Related Traits in Rice

Archana Thiruppathi, Shubham Rajaram Salunkhe, Shobica Priya Ramasamy · Plants · 2024

The Role of Genetic Resistance in Rice Disease Management

Andrews Danso Ofori, Tengda Zheng, John Kwame Titriku · International Journal of Molecular Sciences · 2025

Dissecting Stress-tolerant Traits of CRISPR-edited Crops by High-throughput Omics Technologies

Rinkee Kumari, Ekta Pandey, Shahla Faizan · CABI Biotechnology Series · 2025

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