Application of Box-Behnken Design for the Optimization of Citric Acid Production from Corn Starch Using Aspergillus niger
N. A. Amenaghawon, K. I. Nwaru, F. A. Aisien, S. E. Ogbeide, C. O. Okieimen
Biotechnology Journal International · pp. 236–245 · Published 5 Apr 2013
10.9734/BBJ/2013/3379Abstract
Citric acid production from hydrolysed corn starch was optimized in this study. Response surface methodology (RSM) was employed for the analysis of the simultaneous effect of substrate concentration, broth pH and fermentation temperature on the concentration of citric acid produced during fermentation of hydrolysed corn starch. A three-variable, three-level Box-Behnken design (BBD) comprising 15 experimental runs was used to develop a second degree statistical model for the optimisation of the fermentation conditions. The optimal fermentation conditions that resulted in the maximum citric acid concentration were substrate concentration; 50 g/L, broth pH; 2.00 and fermentation temperature; 25ºC. Under these conditions, the concentration of citric acid was obtained to be 31.96 g/L. Validation of the model indicated no difference between predicted and observed values.
References (18)
- 1 Response Surfaces: Designs and Analyses [DOI]
- 2 Advances in citric acid fermentation by Aspergillus niger: Biochemical aspects, membrane transport and modeling [DOI]
- 3 Direct Determination of Citric Acid in Milk with an Improved Pyridine-Acetic Anhydride Method [DOI]
- 4 Glycerol oxidation in a microfluidic fuel cell using Pd/C and Pd/MWCNT anodes electrodes [DOI]
- 5 Biotechnological production of citric acid [DOI]
- 6 Acid hydrolysis of corn stover for biohydrogen production using Thermoanaerobacterium thermosaccharolyticum W16 [DOI]
- 7 Solid-state fermentation for the synthesis of citric acid by Aspergillus niger [DOI]
- 8 Process optimization for antifungal chitinase production by Trichoderma harzianum [DOI]
- 9 Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs [DOI]
- 10 Microbial Production of Citric Acid by Solid State Fermentation of Kiwifruit Peel [DOI]
- 11 Optimisation studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation [DOI]
- 12 Metabolism of citric acid production byAspergillus niger: Model definition, steady-state analysis and constrained optimization of citric acid production rate [DOI]
- 13 Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation [DOI]
- 14 Citric acid production by a novel Aspergillus niger isolate: I. Mutagenesis and cost reduction studies [DOI]
- 15 Comparative studies on citric acid production by Aspergillus niger and Candida lipolytica using molasses and glucose [DOI]
- 16 Modelling and Simulation of Citric Acid Production from Corn Starch Hydrolysate Using Aspergillus Niger [DOI]
- 17 A kinetic model for citric acid production from apple pomac by Aspergillus niger [DOI]
- 18 Optimization of citric acid production by Aspergillus niger : NRRL 567 in various fermentation systems [DOI]
Cited by 79
V. Sridevi, M. Padmaja, A. Sahitya, N. Harsha Vardhan, G. H. Rao · Biotechnology Journal International · 2015
Nazim Abdul Nariman, Tom Lahmer, Peyman Karampour · Frontiers of Structural and Civil Engineering · 2018
T Palani Rajan, Louis D Souza, G Ramakrishnan · Journal of Industrial Textiles · 2014
Amin Shams, Seyyed Ahmad Mirbagheri, Yousef Jahani · Desalination and Water Treatment · 2019
Tunmise Ayode Otitoju, Yuanyuan Ouyang, Dafu Jiang · Journal of Cleaner Production · 2022
Priya Rai, Ashutosh Pandey, Anjana Pandey · International Journal of Hydrogen Energy · 2019
Seyyed Mohammadreza Davoodi, Saba Miri, Satinder Kaur Brar · Journal of Water Process Engineering · 2023
Amin Shams, Seyyed Ahmad Mirbagheri, Yousef Jahani · Desalination · 2019
Wenwen Geng, Yi Lv, Yanan Duan · Scientia Horticulturae · 2022
Ao Gao, Lining Zheng, Shuanglong Wang · Journal of Bioscience and Bioengineering · 2024
Showing 21 of 79 known citations — external sources report more than can currently be individually listed.
Related research
- Microbial Synthesis of Polyhydric Alcohol by Saccharomyces cerevisiae — shares topic coverage
- Physicochemical Properties of Spicy Lactic Fermented Tigernut-milk Drink Monitored during Ambient and Refrigeration Temperature Storage — shares topic coverage
- Influence of Saccharomyces cerevisiae (Baker’s Yeast) on the Fermentation of Ogi-A Nigerian Fermented Food — shares topic coverage
- Effect of Fermentation on Nutrient and Antinutrient Contents of Fermented Whole and Ground African Breadfruit (Treculia africana) Seeds — shares topic coverage
- Effects of Fermentation and Extrusion on the Proximate and Organoleptic Properties of Cowpea-plantain Flour Blends — shares topic coverage
Article metrics
Real usage data collected on this platform.
131
Page views
0
PDF downloads
45
Outbound clicks
79
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.