Antisickling Activity and Membrane Stabilizing Effect of Anthocyanins Extracts from Adansonia digitata L. Barks on Sickle Blood Cells
P. T. Mpiana, F. S. Misakabu, D. S. T. Tshibangu, K. N. Ngbolua, D. T. Mwanangombo
International Blood Research & Reviews · pp. 198–212 · Published 29 May 2014
10.9734/IBRR/2014/10539Abstract
Background: Parts of baobab tree (Adansonia digitata) including especially the barks are commonly used for their medicinal properties. Aims: The aim of this work is to evaluate the antisickling activity of baobab tree barks, which are used in Congolese traditional medicine to manage Sickle Cell Disease. Study Design: Baobab tree barks was extracted with water by maceration. Phytochemical tests were conducted with standard procedures. Antisickling activity and the minimum concentration of extract required to normalize sickled cells was determined by Emmel test. Place and Duration of Study: This work was done at department of chemistry, Science Faculty, University of Kinshasa (DR Congo), between November 2012 and February 2013. Methodology: The barks collected from Adansonia digitata were dried and powdered. A chemical screening was perform and extraction of anthocyanins done. Antisickling activity was evaluated by Emmel test, membrane stability by osmotic fragility test and Fe3+ evolution by following solution absorbance at 630nm. The rate of sickle cell shape normalization was determined at different plant aqueous extract concentrations in order to determine the minimal concentration of extract required to normalize sickle cells. The shape modification was quantitatively evaluated from the values of parameters such as surface, radius and perimeters of sickle blood cells before or after treatment with plant extract using Motic software. Results: The aqueous extract of Adansonia digitata showed an antisickling activity with a maximal normalization rate of 65.7% and a minimal concentration required to normalize sickled cells of 5.0mg/mL. The cell surface, perimeter and radius were significantly different before and after treatment with plant extract. The chemical screening showed the presence of polyphenols among which anthocyanins. The biological activity of this plant would be due to these pigments. The anthocyanins extract have also shown a stabilization effect on sickle blood red cells membranes and a reduction of methemoglobin to hemoglobin effect Conclusion: The results obtained show significant antisickling activity of Adansonia digitata barks thus justifying the use of this plant by traditional healers in Congolese traditional medicine in the management of Sickle Cell Disease.
References (15)
- 1 THE OSMOTIC RESISTANCE (FRAGILITY) OF HUMAN RED CELLS 1 [DOI]
- 2 The in vitro antisickling and antioxidant effects of aqueous extracts Zanthoxyllum heitzii on sickle cell disorder [DOI]
- 3 In vitro effects of NIPRISAN (Nix‐0699): a naturally occurring, potent antisickling agent [DOI]
- 4 Ethnopharmacological survey of plants used against diabetes in Kisangani city (DR Congo) [DOI]
- 5 In vitro Antisickling Activity of Anthocyanins Extract of a Congolese Plant: Alchornea cordifolia M. Arg. [DOI]
- 6 Antisickling Activity of Medicinal Plants Used in the Management of Sickle Cell Disease in the Tshopo District, DR Congo
- 7 In Vitro Sickling Inhibitory Effects and Anti-Sickle Erythrocytes Hemolysis of Dicliptera colorata C. B. Clarke, Euphorbia hirta L. and Sorghum bicolor (L.) Moench [DOI]
- 8 Pharmacological screening of some traditionally-used antimalarial plants from the Democratic Republic of Congo compared to their ecological taxonomic equivalence in Madagascar [DOI]
- 9 Anti Sickle Erythrocytes Haemolysis Properties and Inhibitory Effect of Anthocyanins Extracts of Trema orientalis (Ulmaceae) on the Aggregation of Human Deoxyhemoglobin S in vitro [DOI]
- 10 In vitro antisickling activity of anthocyanins extracts from Morinda lucida Benth (Rubiaceae).
- 11 Antisickling Activity ofAdansonia digitata [DOI]
- 12 Effects of aqueous extracts of Zanthoxylum macrophylla roots on membrane stability of human erythrocytes of different genotypes [DOI]
- 13 Antisickling activity of anthocyanins of Jatropha curcas L.
- 14 In vitro antisickling activity of anthocyanins extracts of Vigna unguiculata (L.) Walp.
- 15 The nutritive value of Baobab fruit (Adansonia digitata).
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