Antioxidant and Antimicrobial Properties of Selected Plant Leaves
B. O. T. Ifesan, J. F. Fashakin, F. Ebosele, A. S. Oyerinde
European Journal of Medicinal Plants · pp. 465–473 · Published 22 Jun 2013
10.9734/EJMP/2013/3383Abstract
Aims: To determine the antimicrobial and antioxidant potentials of some readily available plant leaves in order to source for alternate antioxidants and antibiotics. Study Design: Randomized complete block design. Place and Duration of Study: Department of Food Science and Technology, Federal University of Technology Akure Nigeria between Feb 2010 and Jan 2011. Methodology: Ethanol, hexane and water extracts from leaves of Anacardium occidentale (cashew), Cocos nucifera (coconut), Citrus sinesis (sweet orange), Citrus limon (lemon) and Carica papaya (pawpaw) were prepared and screened for their antioxidant and antimicrobial properties. The total phenolic content (TPC) was determined by folin-Ciocalteu assay. Antioxidant property of the plant extracts were evaluated using inhibition of free radical 2, 2- diphenyl-2-picryl hydrazyl (DPPH). The antimicrobial activity of the extract against microorganisms (Acinetobacter spp., Bacillus cereus, Escherichia coli, Shigella dysenteriae, Staphylococcus aureus, Salmonella typhi, Aspergillus niger and Aspergillus flavus) was determined using modified agar-well diffusion method. Results: Total phenol content (TPC) of leaf extracts based on tannic acid equivalent revealed that the TPC of cashew leaf ranged from 2.21 to 7.49 mg TAE/g, coconut leaf extract (0.59-2.22 mg TAE/g), lemon leaf extract (0.97-3.9 mg TAE/g), sweet orange (0.54-0.69 mg TAE/g) and pawpaw leaf extract (0.22- 0.36mgTAE/g). At 0.2mg/ml concentration, the highest antioxidant activity was observed from hexane extracts (45.03%-76.05%) followed by water extracts (45.82% -71.7%) and ethanol extracts (32.75%-56.79%). Ethanol extract (0.2mg/ml) from A. occidentale and C. papaya showed antimicrobial activity against all the eight microorganisms tested with inhibition zones ranging from 2-12 mm. The highest inhibition zone of 12mm was observed in A. occidentale leaf against Shigella dysenteriae while C. limon leaf had the lowest inhibition zone of 2 mm against B. cereus. Conclusion: We may conclude that A. occidentale and C. papaya leaves demonstrated broad spectrum activities. The results provided evidence that the plant leaves investigated in this study might indeed be potential sources of natural antioxidant and antimicrobial agents if further investigated.
References (18)
- 1 Antioxidant activity of Nigella sativa essential oil [DOI]
- 2 Free radicals, oxidative stress, and antioxidants in human health and disease [DOI]
- 3 Antioxidant properties of several commercial mushrooms [DOI]
- 4 In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies [DOI]
- 5 Subcritical Water Extraction of Antioxidant Compounds from Rosemary Plants [DOI]
- 6 Chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima Burm. and Citrus sinensis (L.) Osbeck essential oils and their cyclic monoterpene, dl-limonene [DOI]
- 7 Polyphenols in red pepper [Capsicum annuum var. aviculare (Tepin)] and their protective effect on some pro-oxidants induced lipid peroxidation in brain and liver [DOI]
- 8 Change in the Ascorbic Acid, Total Phenol and Antioxidant Activity of Sun-dried Commonly Consumed Green Leafy Vegetables in Nigeria [DOI]
- 9 Chemical constituents and antioxidant activity of Alstonia boonei [DOI]
- 10 Mechanism of potent antiperoxidative effect of capsaicin [DOI]
- 11 Structure-antibacterial activity relationships of anacardic acids [DOI]
- 12 Lemons: Diversity and Relationships with Selected Citrus Genotypes as Measured with Nuclear Genome Markers [DOI]
- 13 Natural amphiphilic phenols as bioactive compounds
- 14 Inhibitory Effect of Eleutherine americana Merr. Extract on Staphylococcus aureus Isolated from Food [DOI]
- 15 The mode of antistaphylococcal action ofEleutherine americana [DOI]
- 16 Antibiotics and Chemotherapy [DOI]
- 17 The use of natural antioxidants in food products of plant origin [DOI]
- 18 The use of natural antioxidants in food products of animal origin [DOI]
Cited by 119
Bihon Abera, Yadessa Melaku, Kebede Shenkute · Applied Biological Chemistry · 2024
Jacinta N. Omeke, Paulinus E. Emennaa, John I. Ihedioha · Comparative Clinical Pathology · 2023
Leila Mehdizadeh, Mohammad Moghaddam, Ali Ganjeali · South African Journal of Botany · 2024
Muluken Adela Alemu, Tilaye Arega Moges, Tirsit Ketsela Zeleke · Scientific Reports · 2025
Abhishek Das, Dipankar Chaudhuri, Rhitajit Sarkar · Nutritional Antioxidant Therapies: Treatments and Perspectives · 2017
Wisuwat Thongphichai, Veerachai Pongkittiphan, Areerat Laorpaksa · Foods · 2023
Kondeti Deep Chandu, Thattantavide Anju, Kanwaljeet Singh · 2021
Mrityunjoy Acharjee, Nagma Zerin, Touhida Ishma · SSRN Electronic Journal · 2021
Kemi Akinwunmi, Esther Odunsi, Chizi Amadi · Journal of Herbs, Spices & Medicinal Plants · 2021
Naif M. Alhawiti, Agnes K. Nthenge, Waeel H. Alramadhan · Advances in Microbiology · 2024
Showing 20 of 119 known citations — external sources report more than can currently be individually listed.
Related research
- In vitro Antioxidant and Anticancer Activity of Cyanobacteria — shares topic coverage
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- A Selective Review of Lipid Autoxidation and Rancidity, Antioxidants, and Dithiooxamide — shares topic coverage
- Antioxidant and Haemato-Biochemical Enhancing Properties of Daucus carota in Male Wistar Rats Toxicated with Lead Chloride — shares topic coverage
- Radical Scavenging Capacity and Efficacy of Myristica fragrans (Nutmeg) Metabolites on Cladosporum herbarum of Food Origin — shares topic coverage
Article metrics
Real usage data collected on this platform.
55
Page views
0
PDF downloads
5
Outbound clicks
119
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.