Antioxidant Activity-Guided Isolation of Phenolic Compounds from Leaves of Vitex pinnata (Lamiaceae)
Hudia Umami Faisal, Mai Efdi, Afrizal Itam, Tia Okselni, Lenny Anwar
Chemical Science International Journal · pp. 1–11 · Published 31 Dec 2022
10.9734/CSJI/2022/v31i6824Abstract
The aim of this study is to isolate phenolic compounds from the ethyl acetate fraction of the leaves of V. pinnata Linn, which have antioxidant properties, guided by a TLC-Bioautography (Thin-layer chromatography) with a reagent of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The TLC-antioxidant bioautography technique has several advantages. It does not require a long time in testing, the sample used is small, and it can potentially obtain promising antioxidant-positive compounds.The isolation process led to obtaining two phenolic compounds. The UV-Vis, FTIR, and NMR spectrum analysis showed that the compounds were (1) p-hydroxybenzoate and (2) luteolin. Further antioxidant evaluation by DPPH assay showed a potential radical scavenging DPPH of luteolin (2) with an IC50 of 1.56±0.18 µg/mL.
Cited by 2
Safa Abdelbaset, Iriny M. Ayoub, R. Tawfiq · Frontiers in Pharmacology · 2026
Zil Arifah, Mai Efdi, Syafrizayanti Syafrizayanti · Journal of The Indonesian Society of Integrated Chemistry · 2024
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