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Research Article Open access CC BY 3.0

Antinociceptive Effect of Ethanolic Extract of Hybanthus enneaspermus Leaf in Rats

A. O. Afolabi, E. T. Oluwakanmi, H. M. Salahdeen, A. O. Oyekunle, I. A. Alagbonsi

Journal of Advances in Medicine and Medical Research · pp. 322–330 · Published 23 Sep 2013

10.9734/BJMMR/2014/4217

Abstract

Aims: To test the hypothesis that Hybanthus enneaspermus leaf has an antinociceptive effect. Methodology: Seventy-two male rats were randomly divided in a blinded fashion into 4 groups each for the tail immersion test (n=12 per group) and formalin test (n=6 per group). Group 1 (control) received 0.6 ml of distilled water. Group 2 received 100 mg/kg of acetaminophen (paracetamol). Group 3 and 4 received 500 mg/kg and 1000 mg/kg of ethanolic extract of Hybanthus enneaspermus leaf (EEHE) respectively. Results: In the formalin test, oral administration of 500 mg/kg and 1000 mg/kg EEHE caused inhibitions of 62.48% and 72% in the early phase and 70.54% and 78.63% in the late phase respectively. The 1000 mg/kg dose significantly reduced the paw licking time when compared to the standard drug (acetaminophen) in the formalin test. The 500 mg/kg and 1000 mg/kg doses significantly increased the tail flick latency in a manner comparable to acetaminophen. Conclusion: This study showed that the leaf has an anti-nociceptive effect.

Acetaminophen analgesic formalin test Hybanthus enneaspermus pain tail flick test

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