Antimicrobial Susceptibility Pattern and Molecular Identification of Acinetobacter baumannii in Alex Ekwueme-Federal University Teaching Hospital Abakaliki, Nigeria
Ikechukwu Herbert Egwu, Ifeanyichukwu Romanus Iroha, Modesta Mmaduabuchi Egwu-Ikechukwu, Ikemesit Udeme Peter, Charity Chinyere Nnabugwu, Chioma Margaret Ali, Elom Emeka Elom, Lillian Ngozika Ogene, Christiana Inuaesiet Edemekong, Ismaila Danjuma Mohammed
Journal of Pharmaceutical Research International · pp. 409–419 · Published 25 Sep 2021
10.9734/jpri/2021/v33i44B32691Abstract
Background and Objectives: Acinetobacter baumannii, a notorious opportunistic pathogen known to seriously affect debilitated individuals especially intensive care unit (ICU) patients and others with underlying illness, have consistently jeopardized many antibiotics. This study was therefore aimed to ascertain the antimicrobial susceptibility profile and molecularly identify A. baumannii pathogens in Alex Ekwueme-Federal University Teaching Hospital, Abakaliki, Nigeria. Methodology: A total of 385 clinical samples were collected aseptically from debilitated patients and analyzed following standard microbiological procedures. Acinetobacter species was confirmed by Gram staining reaction and biochemical tests. All bacterial isolates were phenotypically screened for multidrug resistance using the Kirby–Bauer disc diffusion technique and results interpreted as per CLSI criteria. A. baumannii isolates were finally confirmed using 16S rRNA sequencing. Results: A total of 23(6%) A. baumannii isolates were recovered from 385 clinical samples collected from 87 patients comprising 48 males and 39 females admitted in various hospital wards of AE-FETHA. The age of the patients varied from 20–79 years. The commonest sites for isolation of A. baumannii pathogen were catheter urine (8/8%) and wound sores (7/8%). The highest percentage resistance was observed with cefuroxime (96%), tetracycline (96%), sulfamethoxazole/trimethoprim (96 %), and ofloxacin (91%) while meropenem (91%) and imipenem (78%) were the most effective antibiotics against A. baumannii. The isolated A. baumannii was re-confirmed genotypically by 16S rRNA gene amplification. Variations were observed in the gene sequence of all the isolated A. baumannii. Conclusion: Catheter urine, wound sores, and respiratory fluids were the more easily colonized samples. Also, high frequency of multidrug resistance observed in this study further established A. baumannii as a notorious opportunistic pathogen.
Cited by 3
3 citations reported by external sources — individual citing-article records aren't available to list yet.
Related research
- Molecular Characterization of ESBL and AmpC β-Lactamases among Blood Isolates of Klebsiella pneumoniae and Escherichia coli — shares topic coverage
- Antibacterial Activity of Composite Mixture of Senna siamea Leaves and Tamarind Pulp Extracts on Multidrug Resistant Salmonella typhi — shares topic coverage
- Molecular Characterisation of Enteric Bacteria in Diarrhoeic Stool Samples of Children Less than Five Years in Port Harcourt, Nigeria — shares topic coverage
- Isolation and Identification of Resistant Bacteria from Petroleum Producing Vicinity by Gene Amplification and Sequencing — shares topic coverage
- Morpho-Molecular Characterization of Bacterial Endophytes from Different Banana (Musa sp.) Cultivars across Tamil Nadu, India — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
3
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.