Antifungal Activity of Citral by Disruption of Ergosterol Biosynthesis in Fluconazole Resistant Candida tropicalis
Janiere Pereira de Sousa, Aratã Oliveira Cortez Costa, Maria Clerya Alvino Leite, Felipe Queiroga Sarmento Guerra, Viviane Araújo da Silva, Camilla Pinheiro de Menezes, Fillipe Oliveira Pereira, Edeltrudes Oliveira Lima
International Journal of TROPICAL DISEASE & Health · pp. 1–11 · Published 16 Oct 2015
10.9734/IJTDH/2016/21423Abstract
A limited number of antifungals and the emergence of resistant strains have hindered the treatment of candidiasis, making the search for new antifungals urgent. Citral is a monoterpene with known pharmacological properties, including antimicrobial action. Aims: Thus the objective of this study was to investigate the possible mechanism of citral’s action against Candida tropicalis isolated from human blood, and the effect of its association with antifungals. Methodology: The minimum inhibitory concentration (MIC), the minimum fungicidal concentration (MFC), the effect of citral on the cell wall (sorbitol assay), and membrane ergosterol binding were evaluated using broth microdilution technique. We also evaluated interference in ergosterol biosynthesis, and the citral-antifungal association effect (checkerboard method). Results: The MIC90 and MFC90 of citral were respectively 512 and 1024 μg / mL. The MIC of citral did not increase when sorbitol or ergosterol was added to the medium, suggesting that citral does not act on the cell wall or by membrane ergosterol binding. However, citral inhibited ergosterol biosynthesis, and the citral-fluconazole combination showed synergistic effects for the ATCC strain. Conclusion: This study contributes to understanding the antifungal mechanism of citral’s action, and to the development of new therapies for candidiasis treatment.
Cited by 20
Camilla Pinheiro de Menezes, Ana Luiza Alves de Lima Perez, Janiere Pereira de Sousa · Anales de Biología · 2022
Jaqueline Oliveira Barreto, Larissa Alves da Silva, Luanna de Oliveira e Lima · Archives of Oral Biology · 2025
Mustapha Abubakar, Bhupendra Koul, Yogita Sharma · Phytochemistry Reviews · 2025
Saúl Redondo-Blanco, Javier Fernández, Sara López-Ibáñez · Journal of Food Protection · 2020
Poonam Kumari, Neha Arora, Apurva Chatrath · ACS Omega · 2019
Gleycyelly Rodrigues Araújo, Palloma Christine Queiroga Gomes da Costa, Paula Lima Nogueira · BioTech · 2024
Zhisi Zhang, Xueping Liu, Zhibin Shen · BioMed Research International · 2022
Japheth Omollo Ombito, Godloves Fru Chi, Jean Duplex Wansi · Journal of Ethnopharmacology · 2021
Paula Lima Nogueira, Danielle da Nóbrega Alves, Palloma Christine Queiroga Gomes da Costa · Fungal Biology · 2023
Jeremias Justo Emídio, David Henrique Xavier Barbosa, Ricardo Dias de Castro · Chemistry & Biodiversity · 2025
Showing 19 of 20 known citations — external sources report more than can currently be individually listed.
Related research
- Glycemic Control and Self-Monitoring of Blood Glucose in Type 2 Diabetic Patients on Insulin and Prevalence of Candidiasis in these Patients at Dschang District Hospital, Cameroon: A Cross-Sectional Study — shares topic coverage
- Pediatric Invasive Gastrointestinal Fungal Infections: Causative Agents and Diagnostic Modalities — shares topic coverage
- Oral Candidiasis: Complication of Concomitant Chemo-radiotherapy in Patients with Oral Squamous Cell Carcinoma — shares topic coverage
- Oropharyngeal Candidiasis in HIV Suspected Patients Attending State Hospital Ijebu-Ode Ogun State Nigeria — shares topic coverage
- In vitro Evaluation of Antifungal Activity of Virgin Coconut oil and White Palm Kernel Oil on Candida Species-Experimental Study — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
20
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.