Antidiabetic and Hepatoprotective Activity of Barleria grandiflora Ethanolic Leaf Extract in STZ-induced Diabetic Rats

Abstract

Aim: Diabetes mellitus is one of the most common diseases and a continueing global epidemic, with over 1 to 9 adults living with the diabetes. It exacerbates multiorgan damages including the liver. Our previous study shows potent antioxidant and anti-inflammatory activity in the ethanolic leaf extract of B. grandiflora (BGEE). The present study was designed to evaluate the antidiabetic and hepatoprotective activity of BGEE against diabetic rats. Study Design: BGEE was subjected to evaluate antidiabetic and hepatoprotective activity against STZ-induced diabetic rats. Place and Duration of Study: “Shri Shankaracharya College of Pharmaceutical Sciences, Shri Shankaracharya Professional University, Bhilai-490020, Chhattisgarh, India”, over the period of 4 months. Methodology: For the study, Wistar albino rats (150-180 g) were randomly divided into different groups (n = 6 animals per group). Diabetes was induced in animals by administration of a single dose of STZ (50 mg/kg, i.p.). BGEE at two dose levels, 100 and 200 mg/kg/day, p.o., were administered in their respective groups (BGEE-100 and BGEE-200) for consecutive ten weeks and assessed against the STZ control group. The normal group was also included in the study. Results: BGEE treatments significantly (P < 0.05) reduced blood glucose levels compared to the STZ control group. Moreover, BGEE treatments significantly (P < 0.05) restored the serum hepatic enzymes (SGOT, SGPT, and ALP), hepatic antioxidants (CAT, SOD, and GSH), and lipid peroxidation (MDA) compared to the STZ control group. The beneficial effect of BGEE against diabetic hepatopathy was also reflected in histopathological studies. Conclusion: According to these findings, B. grandiflora is a very promising natural resource for the creation of potent, multi-target phytotherapeutics that can treat glycemic dysregulation and related diabetic hepatopathy.