Antibiogram Analysis of Escherichia coli Isolates Recovered from Dog Faeces

Abstract

Antimicrobial resistance is a global problem affecting animals and humans. Improper use of antibiotics by humans, inefficient prevention and control of infections, poor hygiene and sanitation, are causes for the emergence and distribution of antibiotic resistant bacteria. The role of pets is quite significant as disseminators of antimicrobial-resistant bacteria. This study was planned to analyze the antibiotic susceptibility profile of Escherichia coli isolated from dog faeces. The dog faecal samples (n=5) collected from Veterinary Clinical Complex, College of Veterinary and Animal Science, Navania were subjected to bacteriological examination for the isolation and identification of Escherichia coli. The isolates were confirmed by PCR to detect the species specific uspA gene. Antimicrobial susceptibility testing was done to determine the antibiotic susceptibility pattern of the isolates. In the present study, it was revealed that out of 5 dog faecal samples analyzed, E coli was isolated from 4 samples. The PCR analysis revealed a specific amplification of 884 bp product of the uspA gene among the isolates. Further on analyzing the antibiogram, it was found that chloramphenicol, oxytetracycline, azithromycin, ampicillin, co-trimoxazole and ceftriaxone were effective against E. coli. While, the isolates were resistant to amoxicillin and trimethoprim. Therefore, it was concluded that the screening of cultures by antimicrobial susceptibility testing will not only help in deciding the right antibiotic for treatment but also identify the shifting trends of antimicrobial resistance.