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Research Article Open access CC BY 4.0

Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC

Oludele O. Odekanyin, Temitope T. Aibinu, Olutayo Abioye

Biotechnology Journal International · pp. 1–9 · Published 20 May 2017

10.9734/BJI/2017/31313

Abstract

Aim: This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from the fruit pulp of Trilepisium madagascariense DC. Methods: Crude lectin was obtained by maceration of the fruit and ammonium sulphate precipitation. Hemagglutinating activity assay was carried out by determining the ability of the crude extract to agglutinate red blood cells, serial dilution of the crude lectin was done followed by addition of erythrocytes. The crude lectin was subjected to evaluation for inhibition of bacterial growth by the agar well diffusion method against fourteen human pathogenic gram-positive and gram-negative bacteria. Results: The extract agglutinated trypsinized and glutaraldehyde-fixed rabbit and human erythrocytes with higher specificity for rabbit erythrocytes and better agglutination with trypsinized erythrocytes. Among the various sugar tested, the hemagglutination was best inhibited by galactose. The crude lectin demonstrated mild spectrum antibacterial activity against four pathogenic gram-positive and gram-negative bacteria. The minimum inhibitory concentration ranged from 1.56 mg/ml to 6.25 mg/ml for Streptococcus faecalis, Bacillus anthracis, Staphylococcus aureus and Pseudomonas fluorescens. Conclusion: The study showed that there is presence of hemagglutinins in the extract  and the extract possess antibacterial potential against some pathogenic bacteria tested and can be developed as alternative antibacterial drug that could be employed for the treatment of infectious diseases caused by these pathogens.

Antibacterial lectin pathogen Trilepisium madagascariense hemagglutination infectious disease

References (31)

  1. 1 Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion [DOI]
  2. 2 Antibacterial membrane attack by a pore-forming intestinal C-type lectin [DOI]
  3. 3 Lectins: production and practical applications [DOI]
  4. 4 Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn) [DOI]
  5. 5 Antimicrobial activities of Moringa oleifera Lam leaf extracts [DOI]
  6. 6 Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity [DOI]
  7. 7 Molecular and structural basis for N-glycan-dependent determination of glycoprotein fates in cells [DOI]
  8. 8 Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells [DOI]
  9. 9 Antifungal and antibacterial activities of lectin from the seeds of Archidendron jiringa Nielsen [DOI]
  10. 10 Larvicidal activity of lectins from Myracrodruon urundeuva on Aedes aegypti [DOI]
  11. 11 Bioactive properties of plant species ingested by chimpanzees (Pan troglodytes schweinfurthii) in the Kibale National Park, Uganda [DOI]
  12. 12 Isolation and partial characterization of a novel lectin from Talisia esculenta seeds that interferes with fungal growth [DOI]
  13. 13 PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF LEAF EXTRACT OF PILIOSTIGMA THONNINGII (SCHUM.)
  14. 14 Antifungal and Antiproliferative Activities of Lectin from the Rhizomes of Curcuma amarissima Roscoe [DOI]
  15. 15 Antibacterial Activities of Crude Stem Bark Extracts of Distemonanthus benthamianus Baill [DOI]
  16. 16 A lectin from the rhizomes of turmeric (Curcuma longa L.) and its antifungal, antibacterial, and α-glucosidase inhibitory activities [DOI]
  17. 17 Bio-guided isolation of potential antimicrobial and antioxidant agents from the stem bark of Trilepisium madagascariense [DOI]
  18. 18 Purification, partial characterization and antimicrobial activity of Lectin from Chenopodium Quinoa seeds [DOI]
  19. 19 Antidiarrheal activity of extracts and compound from Trilepisium madagascariense stem bark [DOI]
  20. 20 Inhibition of bacterial adherence to saliva-coated through plant lectins [DOI]
  21. 21 Antimicrobial activity and chemical analysis of some edible oils (Clove, Kalonji and Taramira) [DOI]
  22. 22 THERMAL EXTRUSION and ALKALI PROCESSING of DRY BEANS (PHASEOLUS VULGARIS L.) [DOI]
  23. 23 Galectin-3, a marker for vacuole lysis by invasive pathogens [DOI]
  24. 24 Antimicrobial effects of a crude plant lectin isolated from the stem of Tinospora tomentosa [DOI]
  25. 25 Purification and Identification of a Natural Lectin from the Seed of Peanut Arachis hypogaea [DOI]
  26. 26 The Antimicrobial Potential and Phytochemical Composition of Aristolochia ringens Vahl.
  27. 27 Nutrient, Anti-Nutrients and Phytochemical Compositions of Bosquiae angolensis Fruits "oze" Consumed as Snacks in Enugu State, Nigeria [DOI]
  28. 28 Screening and Partial Purification of Lectin from Various Bangladeshi Plant Seeds [DOI]
  29. 29 Comparative Investigation of the Biological Activity of the Pterocarpus osun and Bosqueia angolensis Seeds Protein Extracts [DOI]
  30. 30 The Useful Plants of West Tropical Africa [DOI]
  31. 31 International Journal of Current Research in Biosciences and Plant Biology [DOI]

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