Skip to content
Research Article Open access CC BY 4.0

Anti-Arthritis, Antioxidant and Anti - Inflammatory Potential of Ethanolic Extract of Guava Leaves on Rats Exposed to High Fat Diet and Freud Adjuvant

Nwosu Patience Chinatu, Ibioku Elekima, Ben-Chioma Adeline, Brown, Holy

Journal of Complementary and Alternative Medical Research · pp. 46–53 · Published 21 Nov 2023

10.9734/jocamr/2023/v24i3503

Abstract

Guava leaves (Psidium guajava) have been used traditionally for years to treat common ailments such as Diabetes, diarrhoea, and hypertension. This study was designed to determine the anti-arthritic, anti-inflammatory, and antioxidant effect of the ethanolic extract of guava leaf (Psidium guajava) on rats fed with a high-fat diet and induced arthritis using complete Freud Adjuvant. Seventy-two male and female albino rats were used in this study, the rats were grouped into 12 with 6 rats in each group, rats were fed a high-fat diet to cause hyperlipidaemia and induced rheumatoid arthritis by injecting 0.1ml of Complete Freund’s Adjuvant into their right hind paw. Group 1 was fed with normal feed and water, group 2 was made of healthy rats, fed with normal feed, water, and guava leaf extract, group 3 was fed with a high-fat diet and induced arthritis with complete Freund’s Adjuvant (CFA) and water, group 4 received dexamethasone (6.75 mg.kg-1 orally), and groups 5 to 12 received the extract at oral doses of 250 and 750 mg/kg, respectively for a period of 28days. ELISA technique was used to analyse the inflammatory markers, antioxidants; SOD, and MDA, while the lipid profile was on a spectrophotometer. The inflammatory markers TNF-α, IL-6, and C-reactive protein were significantly reduced in test subjects at p<0.05, HDL and SOD had no statically significant difference, while MDA was markedly reduced at p<0.05. This study demonstrates that Psidium guajava extract has significant anti-arthritic and lipid-lowering effects. Psidium guajava leaves can be developed into an alternative anti-arthritis and lipid-lowering treatment.

Antioxidant arthritis anti-arthritis Freud adjuvant guava high-fat diet Inflammatory

References (15)

  1. 1 Journal of Agricultural and Food Chemistry [DOI]
  2. 2 The American journal of clinical nutrition [DOI]
  3. 3 Understanding the dynamics: pathways involved in the pathogenesis of rheumatoid arthritis [DOI]
  4. 4 Chemistry and biochemistry of lipid peroxidation products [DOI]
  5. 5 Disease-modifying antirheumatic drugs are associated with a reduced risk for cardiovascular disease in patients with rheumatoid arthritis: a case control study [DOI]
  6. 6 Antidiarrhoeal activity of Psidium guajava Linn. (Myrtaceae) leaf aqueous extract in rodents [DOI]
  7. 7 Guava leaf extract suppresses osteoarthritis progression in a rat anterior cruciate ligament transection model [DOI]
  8. 8 Effect of the administration of Psidium guava leaves on blood glucose, lipid profiles and sensitivity of the vascular mesenteric bed to Phenylephrine in streptozotocin-induced diabetic rats [DOI]
  9. 9 Psidium guajava leaves decrease arthritic symptoms in adjuvant-induced arthritic rats [DOI]
  10. 10 Antioxidant vitamins and coronary heart disease risk [DOI]
  11. 11 A Systemic Review on Antioxidant and Hepato Protective Effect of Psidium Guajava Leaf and Fruit Extract [DOI]
  12. 12 Evidence-Based Complementary and Alternative Medicine [DOI]
  13. 13 Journal of Drug Delivery and Therapeutics [DOI]
  14. 14 Journal of Complementary and Alternative Medical Research [DOI]
  15. 15 Journal of Diseases and Medicinal Plants [DOI]

Cited by 2

A comprehensive review on guava: Nutritional profile, bioactive potential, and health-promoting properties of its pulp, peel, seeds, pomace and leaves

F. Khan, Saeed Akhtar, Muhammad Qamar · Trends in Food Science & Technology · 2025

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

62

Page views

0

PDF downloads

18

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.