Histological and Hematoloical Assessment of the Effect of Heinsia crinata Extract on Dichlorvos (DDVP) Induced Toxicity in Adult Wistar Rats

Abstract

The aim of this study was to assess the “histological and hematological effect of heinsia crinata extract on Dichlorvos (DDVP) Induced Toxicity on liver of adult wistar rats,Thirty (30) adult male Wistar rats were randomly assigned into five groups with group A being the control. Each group contained 6 rats which was given food and water alongside the administration for a period of 21 days which was done orally after acclimatization. Group B was administered orally 35mg//kg DDVP only, group C was administered 35mg/kg DDVP and 500mg/kg Heinsia crinita extract, group D was administered 35mg/kg DDVP and 250mg/kg Heinsia crinita while group E was administered Heinsia crinita extract only. All rats were sacrificed at the end of the 21days and blood was collected by cardiac puncture for hematological analysis and their Livers harvest, processed and stained with Hematoxylin and Eosin for Histopathological studies. Result show PCV showed no significant change in group B, & E (P > 0.05) and was significantly changed in group D (P < 0.05) compared to the control. Hb values showed no significant change amongst the groups (P > 0.05) as compared to the control. No significant changes were observed for WBC in group C, D & E (P > 0.05) as compared to the control but group B was significantly changed (P < 0.05). MCH did not show any significant change across the groups as compared to the control however MCV and MCHC had a significant change in group D while MCHC also had a significant change in group C. Histologically Dichlorvos induce hepatoxicity and inflammation of liver, characterized by the presence of Kupffer's cells, congestion, necrosis and fatty infiltration. It also show that Heinsia crinata has no effect on Dichlorvos induced toxicity.