Analysis of Genetic Diversity using Molecular Markers among Some Elite Rice Genotypes

Abstract

Microsatellites, also known as SSRs, are one of the most commonly utilised DNA marker types for a variety of applications, including genotypic differences, genetic relationships, QTLs, association mapping, genome mapping, and varietal identification. With the goal of assessing genetic diversity analysis of different varieties to measure the extent of genotypic differences, genetic relationship, and also to broaden the germplasm base of future rice breeding programmes, 40 rice genotypes were analysed for genetic variation using 20 SSR markers in the current study. These markers were discovered to produce polymorphic bands among the population, revealing the population's variability. Several alleles were found using microsatellite markers in this study, ranging from 2 to 5, with an average of 3.75 and 0.71 of average genetic diversity. The average PIC value for the current study was 0.62, indicating that the germplasm included in the study is diversified. This study exhibited that SSR primers such as RM1, RM25425, RM20224, RM18107, RM215, RM 248, and RM 535 with the genetic diversity greater than 0.70 and all of these are more polymorphic in nature in distinguishing the genotypes.