An Enzyme-based Spectrophotometric Method for the Determination of Phenolic Compound (2- Methoxyphenol) Using Peroxidase from Ipomea batata
A. M. Magomya, G. G. Yebpella, U. C. Okpaegbe, H. Ataitiya
Asian Journal of Applied Chemistry Research · pp. 11–18 · Published 15 Nov 2022
10.9734/ajacr/2022/v12i2216Abstract
Phenolic compounds and their derivatives are considered priority pollutants because they are harmful to living organisms even at low concentrations. Due to their toxicity and persistence in the environment, many efforts are been made to develop simple and effective methods for their determination. This study describes a peroxidase-based method for the quantitative determination of a named phenolic compound. The method is based on the oxido-reductase activity of peroxidase in a H2O2/2-Methoxyphenol system. Calibration curve of peroxidase activity (absorbance) against the phenol concentration forms the basis for its quantitative estimation. Factors influencing the reaction were evaluated and optimized; Optimum pH and temperature were 6 and 40°C respectively while optimum reaction time was 6 minutes. The calibration curve for the analyte was linear (R2 =0.998) within the concentration range of 0.01–5 mM. Repeatability of analysis was 3.8% RSD for 7 replicate measurements. Recovery tests for the analyte in water samples gave values between 85.33 – 112%.
References (8)
- 1 Review on Research for Removal of Phenol from Wastewater
- 2 Analytical methodologies for the determination of nitroimidazole residues in biological and environmental liquid samples: A review [DOI]
- 3 Determination of total phenolics and anthocyanin contents in the pericarp of hot chilli pepper (Capsicum annuum L.).
- 4 A role of glycosyl moieties in the stabilization of bitter gourd (Momordica charantia) peroxidase [DOI]
- 5 Biochemical and Spectroscopic Characterization of Morning Glory Peroxidase from an Invasive and Hallucinogenic Plant Weed Ipomoea carnea [DOI]
- 6 Partial purification and biochemical characterization of peroxidase from rosemary (Rosmarinus officinalis L.) leaves [DOI]
- 7 Isolation and Characterization of Phenol-Degrading Microorganism: Recent Advances [DOI]
- 8 Determination activities of optimal conditions of peroxidase in hot Pepper and Broccoli [DOI]
Cited by 0
No indexed citations yet.
Related research
- Isolation and Identification of Glucose Oxidase Hyper Producing Strain of Aspergillus niger — shares topic coverage
- Comparative Study of the Alkylsulphatase Activity of Bacteria Found in Soil Contaminated with Detergent in Ondo State, Nigeria — shares topic coverage
- Assessment of Surfactant Degrading Potential of Fungal Isolates from Detergent Contaminated Soil in Ondo State, Nigeria — shares topic coverage
- Characterisation of Some Selected Bacterial Isolates from Vegetable Oil Contaminated Soil — shares topic coverage
- Isolation and Enzymatic Activity of Thermo-tolerant Bacteria from Waste Dumpsites in Umudikeand Environs — shares topic coverage
Article metrics
Real usage data collected on this platform.
10
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.