Adventitious Bud Formation and Plantlet Regeneration of Achyrocline satureoides-A Multipurpose Medicinal Plant
D. Kotik, P. Sansberro, C. Luna
European Journal of Medicinal Plants · pp. 1200–1209 · Published 30 Jun 2014
10.9734/EJMP/2014/8800Abstract
Adventitious bud regeneration was achieved from hypocotyls, cotyledons and leaf explants of Achyrocline satureioides. Organogenesis was induced from every explant cultured on Murashige and Skoog semisolid medium (plus sucrose 30g·L-1) containing different combinations of 6-benzyladenine (BA) and a-naphtalenacetic acid (NAA) under 116mmol·m2·s-1 photosynthetic photon flux density (PPFD), photoperiod 14 h and at 27±2ºC. The regeneration was similar for every tested explant and varied between 64 and 83%. The number of buds formed per regenerative explants was similar in every treatment (5-8 shoots/explant). In order to stimulate In vitro rooting, regenerative leaves were sub cultured from the best induction medium in MS lacking plant growth regulators for the same periods. Every plantlet raised In vitro was phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plot with 60-day-old in vitro regenerated plants was established.
Cited by 2
J. Guariniello, J. Iannicelli, P. A. Peralta · Ornamental Horticulture · 2018
J. Iannicelli, J. Guariniello, S. Alvarez · 2018
Related research
- In vitro Meristem Culture for Rapid Regeneration of Papaya Plantlets in Liquid Media — shares topic coverage
- An Effective Protocol for Carnation (Dianthus caryophyllus L.) cv. 'Geolei' Explants Sterilization for Successful Callusing and Shoot Regeneration — shares topic coverage
- Trend and Structure of Populations of Balanites aegyptiaca in Parkland Agroforestsin Western Niger — shares topic coverage
- Optimization of Base Oil Regeneration from Spent Engine Oil via Solvent Extraction — shares topic coverage
- Effects of Process Variables and a Comparative Study of Methods for Transfer Oil Production from Spent Engine Oil — shares topic coverage
Article metrics
Real usage data collected on this platform.
11
Page views
0
PDF downloads
0
Outbound clicks
2
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.