Activity of Hydroethanolic Leaf Extract of Tecoma stans against Breast Cancer Cells Line - MCF - 7

Abstract

Background: Cancer is abnormal growth of cells, which invade or spread to other parts of the body. There are over 200 types of cancers. Breast cancer is a cancer which develops in tissues of the breast, most commonly originating from the inner lining of milk ducts or lobules that supply ducts with milk. Tecoma stans commonly known as trumpet flower is a yellow trumpet shaped flower belonging to Bignoniaceae, and is native to Americas. It has anti cancer and anti proliferative properties. Aim: Aim of the is to analyse the anti cancer activity of Tecoma stans against breast cancer cells MCF-7. Materials and Methods: Breast cancer cell line (MCF-7) was purchased from National Centre for Cell Sciences (NCCS), Pune, India. Cell viability test was done by MTT assay. BcL2 and BclxL gene expression analysis was done by Real Time-PCR. The obtained data were analysed statistically by one-way analysis of variance and Duncan’s multiple range test with Graph Pad Prism version 5 to analyse the significance. The significance was considered at p<0.05 level in Duncan’s test. Results: The results suggest Bcl2 mRNA gene expression is reduced on induction of 400, 500 ug/ml of Tecoma stans with significant difference in comparison with control. Bcl-xL mRNA gene expression is slightly reduced on induction of 400, 500 μg/mL of Tecoma stans with no significant difference in comparison with control. Conclusion:  From the obtained results, it can be concluded that hydroethanolic leaf extract of Tecoma stans possess anticancer activity against breast cancer cells with respect to Bcl2 mRNA gene expression as compared to Bcl xL mRNA gene expression. If complete research is done, we can use this drug to treat various cancers.