Skip to content
Research Article Open access CC BY 3.0

Abilities of Trichoderma Species to Persist within Maize (Zea mays) Stem Long after Inoculation

A. A. Sobowale, O. O. Babalola, A. D. V. Ayansina, A. O. Obisesan

Microbiology Research Journal International · pp. 95–103 · Published 16 Aug 2011

10.9734/BMRJ/2011/532

Abstract

Different Trichoderma species were examined for their abilities to persist within the maize (Zea mays) stem at different points above and below inoculation points. Different Trichoderma species were isolated from different parts of the maize (Z. mays) plant and its rhizosphere. They were later sent to International Mycological Institute, England for identification. Maize seeds (DMR-LSRW) were planted in pots in the screenhouse. Four weeks after planting, each of the Trichoderma species was inoculated into the stems of the potted plants at the 2nd internodes using the toothpick method. Toothpicks dressed with sterile distilled water served as control. Cut sections of the inoculated stems were examined for presence or absence of the inoculated Trichoderma species at different points far from the inoculated point in the upper and lower internodes after 2, 3, 4, 5 and 6 weeks of inoculation. Ten Trichoderma species were identified; these include five strains of T. pseudokoningii, three strains of T. harzianum, T. hamatum and T. longibrachiatum. All the Trichoderma species were able to move within the stem tissues into the upper and lower internodes. All of them were re-isolated at distant points from inoculation point in the upper and lower internodes even after 6 weeks of inoculation. T. pseudokoningii strain 2 and T. harzianum strains 1 to 3 had the best endophytic movement into the upper and lower internodes. T. hamatum and T. longibrachiatum had the weakest movement into the upper and lower internodes. All the Trichoderma species could thus be said to possess the abilities to persist (endophytic capability) within the maize (Z. mays) stem. T. pseudokoningii and T. harzianum could also be said to be among the best species in the genus Trichoderma with good prospect of biocontrol potential.

Trichoderma species T. pseudokoningii T. harzianum lower internodes upper internodes

References (16)

  1. 1 Mechanisms Employed by Trichoderma Species in the Biological Control of Plant Diseases: The History and Evolution of Current Concepts [DOI]
  2. 2 The Soil-Plate Method for Isolation of Fungi from Soil [DOI]
  3. 3 Postharvest Biological Control of Gray Mold of Apple byCryptococcus laurentii [DOI]
  4. 4 Biocontrol of Postharvest Diseases of Apples with Antagonist Mixtures [DOI]
  5. 5 Influence of Inoculation Method on Systemic Fusarium moniliforme Infection of Maize Plants Grown from Infected Seeds [DOI]
  6. 6 Trichoderma harzianum T39 and T. virens DAR 74290 as Potential Biological Control Agents for Phytophthora erythroseptica [DOI]
  7. 7 Seed Treatment with a Fungal or a Bacterial Antagonist for Reducing Corn Damping-off Caused by Species of Pythium and Fusarium [DOI]
  8. 8 Substrate Colonization, Strain Competition, Enzyme Production In Vitro, and Biocontrol of Pythium ultimum by Trichoderma spp. Isolates P1 and T3 [DOI]
  9. 9 Biocontrol of sclerotinia stem rot ( Sclerotinia minor ) in sunflower by seed treatment with Gliocladium virens [DOI]
  10. 10 Development of Strain-specific Primers for a Strain of Gliocladium catenulatum Used in Biological Control [DOI]
  11. 11 Histopathology of Sweet Corn Seed and Plants Infected withFusarium moniliformeandF. oxysporum [DOI]
  12. 12 Persistence ofTrichodermaspecies within maize stem againstFusarium verticillioides [DOI]
  13. 13 Factors influencing the control of Pythium ultimum-induced seed decay by seed treatment with Pseudomonas aureofaciens AB254 [DOI]
  14. 14 Growth inhibition of Fusarium verticillioides (Sacc.) Nirenberg by isolates of Trichoderma pseudokoningii strains from maize plant parts and its rhizosphere
  15. 15 Forage maize. Production and utilisation.
  16. 16 Biological control of microbial plant pathogens [DOI]

Cited by 2

Trichoderma gamsii (NFCCI 2177): A newly isolated endophytic, psychrotolerant, plant growth promoting, and antagonistic fungal strain

K. Rinu, P. Sati, A. Pandey · Journal of Basic Microbiology · 2014

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

80

Page views

0

PDF downloads

36

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.