Studies on the Extraction of DNA from Human: Resourceful Material  for the Identification

Ahmed Kareem Hussein Alatafi; Noor Hassan Jumaa; Mariam Hussein Ali; Zahraa Abd Alamir Ali; Zainab Zanzal Ahmed

doi:10.9734/jalsi/2025/v28i2684

Research Article Open access CC BY 4.0

Studies on the Extraction of DNA from Human: Resourceful Material for the Identification

Ahmed Kareem Hussein Alatafi, Noor Hassan Jumaa, Mariam Hussein Ali, Zahraa Abd Alamir Ali, Zainab Zanzal Ahmed

Journal of Applied Life Sciences International · pp. 46–65 · Published 9 Apr 2025

10.9734/jalsi/2025/v28i2684

Abstract

The extraction of DNA from human samples is a pivotal process in forensic science, medicine, and genetics, serving as a resourceful tool for identification purposes. This study explores various methods and techniques employed in DNA extraction from human tissues, focusing on their efficiency, reliability, and applicability in different contexts. Additionally, the impact of sample quality, degradation, and contamination on DNA yield and integrity is discussed. We compared 5 different methods to isolate DNA from human saliva and cigarette butts and result have been incorporated in form of gel images and UV-Spectrophotometer absorbance readings. Findings are represented in graphs for same sample with different methods.

DNA genetics DNA extraction forensic science identification

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Ahmed Kareem Hussein Alatafi, Noor Hassan Jumaa, Mariam Hussein Ali, Zahraa Abd Alamir Ali, Zainab Zanzal Ahmed (2025). Studies on the Extraction of DNA from Human: Resourceful Material for the Identification. Journal of Applied Life Sciences International , 46–65. https://doi.org/10.9734/jalsi/2025/v28i2684

Affiliations

Ahmed Kareem Hussein Alatafi — Department of Biology, College of Science, University of Diyala, Iraq.
Noor Hassan Jumaa — Department of Biotechnology, College of Science, University of Diyala, Iraq.
Mariam Hussein Ali — Department of Biotechnology, College of Science, University of Diyala, Iraq.
Zahraa Abd Alamir Ali — Department of Biotechnology, College of Science, University of Diyala, Iraq.
Zainab Zanzal Ahmed — Department of Biotechnology, College of Science, University of Diyala, Iraq.

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