Antimicrobial Resistance of Pathogenic Bacteria Isolated from Mastitis Cows in Khartoum State, Sudan

Abstract

Aims: The problem of antimicrobial resistance (AMR) is now recognized as a major threat to the health and development in all countries. This study was conducted to determine pathogenic bacteria associated with clinical cases of bovine mastitis and their antimicrobial resistance patterns. Study Design: This study was carried out In the Department of Bacteriology, Central Veterinary Research Laboratory, Khartoum, Sudan during the period from April 2013 to March 2014. Methodology: 150 milk samples from clinical cases of bovine mastitis were cultured onto blood agar plates and the isolated organisms were identified by conventional bacteriological methods. One hundred and five isolates were tested against 11 antimicrobial agents commonly used in the dairy farms using the disc diffusion method. Results: The majority of the isolates were highly sensitive to gentamycin, ciprofloxacin, norfloxacin and kanamycin, highly resistance to penicillin-G and moderately sensitive to novobiocin, tetracycline and cefalexin. Conclusions: This study revealed that a number of significantly public health concern bacteria were isolated from milk samples and increasingly developed resistance to different groups of antimicrobial agents.