A Synergistic Combination of Cytokinins and Gibberellins Promotes Shoot Regeneration and Proliferation in In vitro Cultured Nodal Explants of Guava (Psidium guajava L.)

Abstract

Guava (Psidium guajava L.) is an important tropical fruit of the Myrtaceae family that was traditionally propagated through seeds, which often led to variations in the seedlings due to natural cross-pollination. Due to the slow multiplication process through vegetative methods, there is a need for the development of an efficient in vitro regeneration technique. The slow multiplication process through vegetative methods necessitates the development of an efficient in vitro regeneration technique. The present study was carried out to investigate the shoot induction from nodal segments that were cultured on a full-strength MS medium supplemented with different concentrations of BAP, kinetin, and GA3.Nodal explants cultured in MS media fortified with BAP 1.0 + GA3 0.25 mgL⁻¹ took the minimum number of days for shoot initiation (25.14 days). The maximum number of shoots (3.14) per explant was observed in the treatment containing BAP 2.0 + GA3 0.5 mgL⁻¹ after 60 days of culture. The highest shooting percentage (71.02 %) with the maximum number of leaves (7.18) was observed in the treatment combination of BAP 1.0 + GA3 0.5 mgL⁻¹ after 60 days of culture. Maximum shoot lengths of 3.92 cm (45 days) and 4.16 cm (60 days) with a longer internodal length of 1.44 cm (60 days) were recorded in the treatment containing the combination of BAP 1.0 + GA3 0.5 mgL-1.