A Study of Prophylactic Effect Against Diabetes of Two Ayurvedic Drugs ‘Jambadyarista’ and ‘Bohumutrantak Ras’ in Normal as well as Alloxan-induced Diabetic Rats

Abstract

Aims: The present study was designed to evaluate the anti-diabetic as well as prophylactic activity of Jambadyarista and Bohumutrantak Ras in alloxan-induced diabetic rats. Study Design: Study the prophylactic and antiglycemic effects against diabetes of two Ayurvedic drugs ‘Jambadyarista’ and ‘Bohumutrantak Ras’ in normal as well as alloxan-induced diabetic rats using in vivo models. Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh between December 2013 and March 2014. Methodology: To investigate the activity, 70 Long Evans rats divided into seven (A-G) groups were included in this study. Diabetes was induced by single intraperitoneal injection of alloxan (150mg/kg body weight) while the Ayurvedic drugs Jambadyarista and Bohumutrantak Ras were given orally at a dose of 200mg/kg of body weight. Group A was control group. Groups B and E were allowed to fast for 12 hours. Diabetes was induced by intraperitoneal injection of freshly prepared solution of alloxan (150mg/kg) in normal saline after base line glucose level determination. The alloxan-treated rats were allowed to food over night to overcome drug induced hyperglycemia. After 48 hours, blood glucose level was measured with an Accu-Chek glucometer using blood sample from the tail vein of each rat. Diabetes was established in animals when blood glucose level was raised to 11.1-32.6mmol/L. After the establishment of diabetes, the experiments were carried out. Groups D and G were allowed to induce diabetes by single intraperitoneal injection of alloxan (75mg/kg body weight) in normal saline every day for 5 days. Results: In case of alloxan-induced diabetic rats, Jambadyarista and Bohumutrantak Ras showed prophylactic activity against diabetes as well as also reduced blood glucose level. By statistical analysis of results, it was found that Jambadyarista and Bohumutrantak Ras have prophylactic activity against diabetes in normal and alloxan- induced diabetic rats. Conclusion: It can be inferred that the Ayurvedic drug “Jambadyarista and Bohumutrantak Ras” significantly possess prophylactic activity against diabetes. They also showed antidiabetic effect in alloxan-induced diabetic rats. Further comprehensive cellular and molecular investigations are required to characterize the exact mechanism responsible for its prophylactic and antidiabetic effects.