Skip to content
Research Article Open access CC BY 3.0

A Comparative Study of Spectrophotometric Methods Versus Chemometric Methods; An Application on a Pharmaceutical Binary Mixture of Ofloxacin and Dexamethasone

Nagiba Y. Hassan, Samia M. Elgizawy, Hayam M. Lotfy, Sarah S. Saleh

International Research Journal of Pure and Applied Chemistry · pp. 90–110 · Published 17 Mar 2013

10.9734/IRJPAC/2013/3052

Abstract

Aim: To conduct a comparative study between the smart novel ratio difference spectrophotometric method (RDSM) versus four spectrophotometric methods: first derivative spectrophotometry (D1), first derivative of the ratio spectra (1DD), isoabsorpative point (Aiso), ratio subtraction (RS), and two chemometric techniques based on principal component regression (PCR) and partial least-squares (PLS-1) for the determination of a binary mixture of Ofloxacin (OFX) and Dexamethasone (DXM). Study Design: The results obtained from the proposed methods were statistically compared to the reported HPLC method using student’s t-test, F-test and One way ANOVA. Methodology: (OFX) was determined by the application of direct spectrophotometry, by measuring its zero-order (D0) absorption spectra at its λmax = 296.6 nm. (DXM) was determined by (D1) at 227.1 nm. By applying (1DD), (DXM) was determined at 237.3. The total concentration of both (OFX + DXM) was determined at their isoabsorpative point λiso= 238.3 nm, then the concentration of (DXM) in mixtures were calculated by subtraction. (DXM) was determined using the (RS) method at its λmax = 239 nm. (DXM) was determined using (RDSM) by measuring amplitude difference at two selected wavelengths (248.4 and 290 nm). A concentration of 10 µg.mL-1 of OFX was used as a divisor. The linearity range was found to be (1-10 µg.mL-1) and (2-14 µg.mL-1) for OFX and DXM respectively. Results: The recovery percentage for OFX was found to be 100.07 ±0.65 and for DXM was found to be 100.41 ±0.84, 100.15 ±0.97, 100.14 ±0.91, 100.54 ±0.75 and 100.11 ±.66 for the five methods, respectively. Conclusion: The novel method showed advantages over the other proposed methods regarding simplicity, minimal data manipulation and maximum reproducibility and robustness; which enabled the analysis of binary mixtures with overlapped spectra for routine quality control testing with quite satisfactory and in lower cost.  

Ofloxacin dexamethasone ratio difference isoabsorpative point ratio subtraction chemometric

References (22)

  1. 1 Chemometrics: Data Analysis for the Laboratory and Chemical Plant
  2. 2 Clarke's analysis of drugs and poisons
  3. 3 A new spectrophotometric method for quantitative multicomponent analysis resolution of mixtures of salicylic and salicyluric acids [DOI]
  4. 4 Comparative study of novel spectrophotometric methods manipulating ratio spectra: An application on pharmaceutical ternary mixture of omeprazole, tinidazole and clarithromycin [DOI]
  5. 5 Smart Stability-Indicating Spectrophotometric Methods for Determination of Binary Mixtures Without Prior Separation [DOI]
  6. 6 Spectrofluorimetric study of the acid–base equilibria and complexation behavior of the fluoroquinolone antibiotics ofloxacin, norfloxacin, ciprofloxacin and pefloxacin in aqueous solution [DOI]
  7. 7 A comparative study between PCR and PLS in simultaneous spectrophotometric determination of diphenylamine, aniline, and phenol: Effect of wavelength selection [DOI]
  8. 8 Chemiluminescence methods for the determination of ofloxacin [DOI]
  9. 9 Spectrophotometric methods for the simultaneous determination of binary mixture of metronidazole and diloxanide furoate without prior separation [DOI]
  10. 10 Simultaneous determination of malondialdehyde and ofloxacin in plasma using an isocratic high-performance liquid chromatography/fluorescence detection system [DOI]
  11. 11 Spectrophotometric determination of benazepril hydrochloride and hydrochlorothiazide in binary mixture using second derivative, second derivative of the ratio spectra and chemometric methods [DOI]
  12. 12 Development and validation of a stability-indicating RP-HPLC method to separate low levels of dexamethasone and other related compounds from betamethasone [DOI]
  13. 13 Determination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection [DOI]
  14. 14 A validated, stability-indicating HPLC method for the determination of dexamethasone related substances on dexamethasone-coated drug-eluting stents [DOI]
  15. 15 Determination of dexamethasone in tears by capillary electrophoresis [DOI]
  16. 16 Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products [DOI]
  17. 17 Simultaneous Determination of Trace Ofloxacin, Ciprofloxacin, and Sparfloxacin by Micelle TLC-Fluorimetry [DOI]
  18. 18 High-performance liquid chromatographic method for the assay of dexamethasone and xylometazoline in nasal drops containing methyl p-hydroxybenzoate [DOI]
  19. 19 Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography [DOI]
  20. 20 New spectrofluorometric application for the determination of ternary mixtures of drugs [DOI]
  21. 21 Chemometrics assisted spectroscopic determination of vitamin B6, vitamin B12 and dexamethasone in injectables [DOI]
  22. 22 HPLC-UV METHOD FOR SIMULTANEOUS DETERMINATION OF OFLOXACIN AND DEXAMETHASONE SODIUM PHOSPHATE Research Article

Cited by 26

Analytical investigation of different mathematical approaches utilizing manipulation of ratio spectra

Essam Eldin A. Osman · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2018

Different mathematical processing of absorption, ratio and derivative spectra for quantification of mixtures containing minor component: An application to the analysis of the recently co-formulated antidiabetic drugs; canagliflozin and metformin

Hayam M. Lotfy, Dalia Mohamed, Mona S. Elshahed · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2018

Comparative Study of the Spectral Resolution Efficiency of the Recently Developed and Conventional Spectrophotometric Methods in the Analysis of Severely Overlapped Zero-Order Absorption Spectra with the Same Geometrical Features

Hayam M. Lotfy, Yasmin Mohammed Fayez, Eman M. Morgan · Analytical Chemistry Letters · 2016

Evaluating the efficiency of spectral resolution of univariate methods manipulating ratio spectra and comparing to multivariate methods: An application to ternary mixture in common cold preparation

Azza Aziz Moustafa, Hesham Salem, Maha Hegazy · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2015

Advanced Approaches in Green Univariate Spectrophotometric Methods

HayamM. Lotfy, SarahS. Saleh, Yasmin Rostom · Sustainable Approaches in Pharmaceutical Sciences · 2023

Novel spectrophotometric methods for simultaneous determination of timolol and dorzolamide in their binary mixture

Hayam Mahmoud Lotfy, Maha A. Hegazy, Mamdouh R. Rezk · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2014

Critical assessment of smart calculation-based spectroscopy versus chemometric-assisted methods: Application to combined antibiotic formulations

Hind Ali Abdullatif, Adel Magdy Michael, Yossra Ahmed Trabik · European Journal of Chemistry · 2022

Trade-off efficacy and data processing strategy in the power of spectral resolution of co-formulated antihypertensive pharmaceuticals

Yasmin Rostom, Hayam M. Lotfy, Meltem Öztürk · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2021

Comparative study of novel versus conventional two-wavelength spectrophotometric methods for analysis of spectrally overlapping binary mixture

Hayam M. Lotfy, Maha A. Hegazy, Mamdouh R. Rezk · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · 2015

Risk assessment based on spectrophotometric signals used in eco-friendly analytical scenarios for estimation of carvedilol and hydrochlorothiazide in pharmaceutical formulation

Mona Nabil, Hoda M. Marzouk, Dina A. Ahmed · Scientific Reports · 2024

Showing 11 of 26 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

77

Page views

0

PDF downloads

43

Outbound clicks

26

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.