In vivo Biochemical Evaluation of Some Synthesize Thiazole Derivatives Containing Coumarin Moiety as Antioxidant and Antitumor Agents

Abstract

Background: Coumarin and thiazole derivatives have been used traditionally for many centuries. Because they have anti-tumor, antioxidant activities, and induced apoptosis. Aim: The present study aims to investigate the in vivo antitumor, and antioxidant activities of potassium salt of 5-(4 -chlorophenyl)-2-[(7-hydroxy coumarin-3-ylethylidene) acetyl hydrazine]-1,3 thiazole (5a) and 5-(4 -chlorophenyl)-2-[(8-hydroxy coumarin-3-ylethylidene) acetyl hydrazine]-1,3 thiazole (5b). Materials and Methods: Toxicity has been determined for the synthesized compound. Anti-cancer and anti-oxidant activities were studied through the evaluation of tumor cell viability, lifespan prolongation and antioxidant estimation. Results: Doses of up to 500 mg/kg showed good protection in 5a and 5b compounds. Results of in vivo anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells for the compounds studied showed a significant reduction in the volume and number of ascites. And increased in life span. In therapeutic and preventive groups, compounds 5a and 5b have anti-oxidant properties by a significant decrease in malondialdehyde and significant increased catalase and GSH. Also, compounds 5a and 5b induced apoptosis by increase Caspase-3 and BCL-2 associated protein (BAX), Compared to the group of positive controls. Conclusion: It was concluded that compounds 5a and 5b have a potent antitumor and antioxidant activity against Ehrlich ascites carcinoma in mice.