Haploid Plant Regeneration from Unpollinated Ovules of Cucumis melo L. Var. Conomon cv. Mudicode

Abstract

Major objective of the present work was to induce haploid plants in Cucumis melo var. conomon cv. Mudicode (Common name: Kani vellari). Ovules of Kani vellari plants were collected one day before anthesis and were cultured in vitro on MS medium supplemented with IAA, IBA, NAA, 2, 4-D, KN, BAP, TDZ and 2-iP (1.0, 2.0 and 5.0 µM) and BAP/TDZ/KN (1.0, 2.0 and 5.0 µM) in combination with IAA, IBA, NAA, 2, 4-D (1.0, 2.0 and 5.0 µM). The cultured ovules involved in callus induction and an optimum quantity of 93.15 mg of callus from cultured ovules was obtained on the MS medium fortified with 1 µM BAP + 2 µM NAA. The callus induced on medium supplemented with 1 µM BAP+2 µM NAA was subcultured on MS medium supplemented with 5 µM BAP was involved in shoot organogenesis and developed a maximum of 5.55 shoots. The rooting of the regenerated shoots was achieved on MS medium supplemented with 1 µM IAA. Cytological analysis of the root tips of regenerated plants confirmed their haploid nature.