Molecular Characterization of ESBLs Genes among Multi-drug Resistant Klebsiella species in Ile-Ife South-West, Nigeria

Abstract

Aim: This study was carried out to determine the presence of ESBLs genes (TEM, CTX-M, SHV, AAC and OXA types of Beta-lactamases) in clinical isolates of Klebsiella species producing extended spectrum beta-lactamase. Extended Spectrum Beta Lactamase (ESBL)-producing Klebsiella have increased rapidly and became a major problem in the area of infectious diseases. Nosocomial infections are primarily caused by Klebsiella bacteria, which leads to an increase in health care costs and mortality rate. Methodology: A total of 166 bacteria was isolated. Resistance to different antibiotics was determined using the standard disk diffusion method. Extended spectrum beta-lactamases were screened for using disc diffusion method, confirmatory test was detected by the double disk synergy test (DDST) method and polymerase chain reaction (PCR) was used to determine blaCTX, blaOXA, blaSHV, AAC and blaTEM genes in the ESBLs positive isolates. Results: blaTEM (31%) was the most prevalent and AAC (27%). The rates of CTX-M and SHV genes are 21% and 13% respectively. Conclusion: Outbreak of isolates producing ESBLs can cause serious problems in the future, regarding the treatment of infections caused by this common pneumonia pathogen.