Microbial Contamination of Environmental Surfaces in An-Najah National University Setting

Abstract

Aims: To investigate bacterial contamination of environmental surfaces in An-Najah National University setting. This study focused mainly on staphylococci bacteria in particular detection of some molecular characterization of Staphylococcus aureus. Place and Duration of the Study: Department of Biology and Biotechnology, An-Najah National University, Palestine, March-August 2012. Methods: A total of 210 environmental surface samples from Faculty of Science, An-Najah National University were collected using cotton swabs. S. aureus, biochemical tests and S. aureus species-specific identification was used to confirm the isolates. Antimicrobial susceptibility testing for methicillin was performed, mecA gene, SCCmec typing and seg, seh and sei toxin genes were detected by PCR. Morphology, biochemical tests and selective media were used to identify other microorganisms obtained from contaminated environmental surfaces. Results: It was found that 31.9% (67/210) of these surfaces were contaminated with S. aureus. Only 5 methicillin resistant S. aureus (MRSA) isolates were detected and belonged to SCCmec IVa type. One isolate of methicillin sensitive S. aureus (MSSA) was enterotoxigenic and had genotype seg+/seh+. In addition, 85.7%, 90.5%, 14.3%, 11.9%, 10% and 4.8% of cultures were positive for S. epidermidis, fungi, Bacillus sp., Escherichia coli, Klebsiella sp. and Streptococcus sp., respectively. Conclusion: The results show that these different representative environmental surfaces are in daily use and may be a source of infection.