Assessment of Genetic Diversity in Some Landraces of Common Beans Using Inter-Simple Sequence Repeat (ISSR)

Abstract

Aims: Use molecular tools to characterize six common beans genotypes, which were collected from distinct geographical origins in Egypt. This is to explore the existence of genetic variability that may help to generate strategies for conservation and potential utilization in future beans breeding programs. Study Design: Completely randomized.  Place and Duration of Study: This investigation took place from June to December 2022 at the laboratory of the vegetable seed of Sabahya Horticulture Research Station, Alexandria Governorate, Agricultural Research Center, Egypt. Methodology: Inter-Simple Sequence Repeat (ISSR) marker was employed to evaluate the efficiency in diversity analysis of common beans genotypes. Results: There were genetical diversity among the six studied genotypes of common beans according to the used four markers of ISSR. Conclusion: It is important to collect local varieties and determine their genetic diversity in order to protect genetic resources and use them in breeding studies. The records obtained in this study make it possible to establish the basis to generate use and conservation strategies for the evaluated common beans genotypes. There is genetic variability among beans genotypes from different geographical locations. ISSR markers were effective in genetically distinguishing the evaluated materials despite their expected high homozygosity due to the autogamous nature of this crop.