Synthesis of Silver Nanoparticles by Sodium Borohydride Reduction Method: Optimization of Conditions for High Anti-staphylococcal Activity
Ifeanyi Nzekwe, Chukwuma Agubata, Chukwuebuka Umeyor, Ifeanyi Okoye, Chidalu Ogwueleka
British Journal of Pharmaceutical Research · pp. 1–9 · Published 10 Jan 2016
10.9734/bjpr/2016/30324Abstract
Aim: The aim of this research was to optimize the reaction conditions for the production of silver nanoparticles sufficiently bioactive for incorporation into pharmaceutical gels. Methods: Silver nanoparticles were prepared by silver nitrate-sodium borohydride reduction method under different stoichiometric conditions and differences in their spectral properties were investigated while bioactivities were studied against different bacterial species. The most bioactive sample was characterized using transmission electron microscopy, atomic force microscopy and photon correlation spectroscopy, and the influences of varying reaction conditions (pH, solvent type and solvent temperature) were investigated using the stoichiometric ratio with best bioactivity. Results: The 25: 25 batch (sodium borohydride and silver nitrate in equal volume ratio) demonstrated the highest bioactivity which was significantly different (P = 0.05) from others. Physicochemical characterization revealed a hydrodynamic size of 17.46 d.nm (pdi 0.62) and a peak resonance mode at 450 nm. Bioactivity was highest at neutral pH conditions of reaction and improved with precursor solvent temperature, up to 45°C. Whereas the control sample of ciprofloxacin demonstrated no activity against Staphylococcus aureus, silver nanoparticles in methanol exhibited very good activity against Staphylococcus aureus. Conclusion: It is hereby concluded that reaction conditions can affect the antimicrobial activity of silver nanoparticles, possibly by influencing the size and yield of silver nanoparticles using the silver nitrate-sodium borohydride reduction method.
Cited by 16
Sadia Mehmood, Naeem Akhtar, Muhammad Nadeem Arshad · International Journal of Biological Macromolecules · 2024
Muhammad Faiz Aizamddin, Mohd Muzamir Mahat, Zaidah Zainal Ariffin · Polymers · 2021
Eva María Mateo, Fernando Mateo, Andrea Tarazona · Toxins · 2025
Mai Xuân Dũng, Quang-Trung Le, Lan-Anh Nguyen Thi · Material Science Research India · 2019
Sarah Constance Motshekga · Chemical Papers · 2022
Rossannie Guasamucare, Emma Parente, Juan Pereira · Journal of Surfactants and Detergents · 2024
Kartick Chandra Majhi, Paramita Karfa, Rashmi Madhuri · Nanotechnology in the life sciences · 2020
Lulu Fang, Honglei Chen, Fangong Kong · Cellulose · 2018
Ifeanyi Thaddeus Nzekwe, Chidinma Okoli, Chinomso McAnthony Ewulonu · BioNanoScience · 2020
Showing 9 of 16 known citations — external sources report more than can currently be individually listed.
Related research
- Reasons Why Students Study Medicine: Views of Students at Delta State University Abraka, South-South Nigeria — shares topic coverage
- Harnessing Artificial Intelligence in Healthcare Analytics: From Diagnosis to Treatment Optimization — shares topic coverage
- Exploring Hemophilic Pseudotumors: A Comprehensive Case Series — shares topic coverage
- A Novel Catalytic Synthesis of Flavones under Autoclave Conditions and Comparative Study of Anti-cancer Activity — shares topic coverage
- HPTLC Densitogram Profile of Aqueous Distillate (Arka) from Roots of Boerhaavia diffusa — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
16
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.