Using Safe Calculated Low Power of Electrons to Cut, Analyze and Exterminate the Outer and Inner Biological Elements of SARS-CoV-2, MERS-CoV-2 and Influenza viruses in Vitro

Abstract

Background: The treatment of pneumonia viruses with antiviral drugs produced mild to severe side effects until the immune system worked against the invaded virus. Objective: Therefore, it is crucial to develop an efficient alternative therapy with lower side effects against infection of pneumonia viruses. Methods: We hereby designated a novel device termed a a Life Restoration Device (LRD to produce electric frequencies with lower potential. The electric frequencies cancan specifically destroy the nucleic acid materials and viral envelope rather than the plasma of the infected cell. Using a glass tube model the infected cells with propagated viruses were filled in the glass tube and two nickel-coated copper rods were inserted into both ends. Then the two rods were connected into the LRD. Lower potential electric frequencies were applied for 30 min and 60 min onto the infected cells. Results: The treatment of the infected cell culture of MERS-CoV and SARS-CoV-2 using LRD for 30 min significantly reduced the viral infectivity to 83% and 22% respectively. After 60 min of MERS-CoV and SARS-CoV-2 exposure to LRD, the infectivity reduced to 21% and 1% respectively. Additionally, based on the data of transmission electron microscopy of the H5N1 virus and electrophoretic patterns of different types of viruses, the nucleic acid materials of the treated viruses were reduced based on the non-treated viruses. The electric frequencies sensitivity produced by LRD can reduce the fluidity of the viral envelope rather than the plasma membrane of the infected cells. Conclusion: Treatment of pneumonia viruses with electric stimulation is a new alternative therapy but needs more investigations. The data obtained from this study is important to develop an effective alternative viral therapy.