Antimicrobial Activity of Pyocyanin for Inhibition of Pseudomonas aeruginosa Urinary Tract Pathogens

Abstract

This study aims to detect the antimicrobial activity of pyocyanin for inhibition of Pseudomonas aeruginosa urinary tract pathogens. Five different isolates of Pseudomonas aeruginosa were isolated from wound infection and eye drops. The isolates were tested for the production of pyocyanin by growing in nutrient agar after incubation at 37°C for 18 hours. Considerable amount of blue pigment pyocyanin were produced by Pseudomonas aeruginosa after growing in lauryl broth medium and extracted by chloroform extraction method. Maximal pigment achieved after 72 hours and used in sensitivity test against isolated microorganisms" S. aureus, S. epidermis, S. saprophyticus, E. coli, K. pneumoniae, P. aeruginosa, C. freundii and C. albicans". The antimicrobial activity of the pigment was evaluated using Mueller Hinton Agar medium. The isolated microbes from urinary tract infection have different percentages of microorganisms" S. aureus 15%, S. epidermidis 5%, S. saprophyticus 10%, E. coli 40%, K. pneumoniae 10%, P. aeruginosa 5%, 5% and C. albicans 10%". The pigment have properties that make it an important bioactive compound which has the ability to arrest the electron transport chain of bacteria and exhibit antibacterial activity towards gram positive and uncapsulated gram negative by different zone of inhibition at different concentrations. The inhibition zone was determined by using 25%, 50%, 75% and 100%. The S. aureus inhibition zone at 25% was 16 mm, 50% was17.5 mm, 75% was 20 mm and 21 mm at 100% concentration, S. epidermidis at 25% was 17 mm, 50% 20 mm, 75% was 21 mm and 31 mm at 100%, S. saprophyticus at 25% was 19 mm, 50% was 23 mm, 75% was 25 mm and 33 mm at 100%. E. coli at 25% was 13 mm, 50% was 15 mm, 75% was 25 mm and 22 mm at 100%. was resistant to 25% and 50% concentration and given inhibition zone 11,13 mm at 75,100% concentration. Also K. pneumoniae, P. aeruginosa, and C. albicans were resistant to pyocyanin. This study concluded that pyocyanin possess antibacterial activity against U T pathogens.