Protective Effect of Dorema glabrum on Diazinon-induced Oxidative Stress in Rat Kidney

Abstract

Introduction: A wide number of pesticides such as diazinon (DZN) have deteriorating effect on fauna and flora by inducing oxidative stress and cell damage. The aim of current study was to investigate the protective effect of D. glabrum against the subchronic effect of DZN on serum urea and creatinine, oxidative damage markers such as lipid peroxidation (LPO) and the antioxidant defense system (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the kidney of male Wistar rats. Methods: Twenty-four adult male Wistar rats randomly divided into four groups including a control group, and three experimental groups. Two of three experimental groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days along with DZN (100 mg/kg) that injected intraperitoneally in the last day of D. glabrum usage, and one group received only DZN (100 mg/kg). Results: The levels of LPO, urea, creatinine, and the decreased antioxidant defenses, like free radical scavenging enzymes viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) significantly increased in DZN-treated group as compared to control group. Distinctly increased LPO levels, with alterations in endogenous antioxidant enzyme were evident in nephrotoxicity of DZN. Specific marker enzymes were restored to normalcy in rats supplemented with D. glabrum following treatment with DZN which otherwise was decreased in the DZN-treated rats. Conclusion: The obtained results revealed that the oxidative stress of rats treated via DZN can be decreased if it is co-treated by D. glabrum with DZN. In addition, this co-treatment might serve a putative protective agent against kidney damage generated by DZN.