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Research Article Open access CC BY 4.0

RT-PCR Mediated Identification of SARS-CoV-2 Patients from Particular Regions of Bangladesh and the Multi-factorial Analysis Considering Their Pre and Post Infection Health Conditions

Kazi Mahmuda Akter, Tanjum Tushi, Sabrina Jahan Mily, Rashenda Aziz Mohona, Samin Anis, Adittya Kishor Chakraborty, Elmee Tabassum, Tahmid Ul Islam, Ozifatun Jannat Akhi, Israt Sultana Nishe, Badrun Nahar Laxy, Sanjida Sultana Zerin, Abdiqani Jama Roble, Md. Imran Hossain, Sharmin Ahmed, Salauddin Al Azad

Biotechnology Journal International · pp. 43–56 · Published 11 Dec 2020

10.9734/bji/2020/v24i630121

Abstract

Background: SARS-CoV-2 is a type of coronavirus shows bizarre features with fatality rates at 34.4%. The clinical and non-clinical features of COVID-19 might be causative factors for more rapid community spreading compared to MERS and SARS-CoV-1. Objectives: To study the efficacy of RT-PCR based detection of SARS-CoV-2 patients. Besides, the patients' pre and post-infection health conditions depending on selective clinical and nonclinical parameters were analyzed. Materials and Methods:  Clinical and non- clinical data were collected from 205 randomly selected COVID-19 patients, identified through RT-PCR from different areas of Bangladesh. The data were analyzed using statistical and analytical tools to illustrate the impact of the pandemic situation on the sufferers.  Results: Molecular identification of the patients through RT-PCR has been observed as the most efficient option for detecting SARS-CoV-2 patients. Patients with the smoking habit have been reported to be more prone to the COVID-19 infection; surprisingly the non-smoker female was the worse suffers. The patients aged 60-69 years were the worst sufferers with every adverse health conditions mentioned. Professionally, 28% self-employed male and 8.19% female were infected. The most infected individuals were house wife (32.029%), as next to the health care workers (22.1%). With the increased age group, a decreased number of smokers were found.               Nebulization of 5% home and 12% hospital treated patients were provided, with Oxygen for 26% of hospitalized patients. After recovery, combined physical and psychological complications were observed in 19% of male and 11% female. The socioeconomic, environmental and geographical annotations exposed a clear relationship with the rate of infection as revealed from the nonclinical data analysis. Conclusion: RT-PCR proved its specialty in COVID-19 detection. In addition, impacts of different clinical and nonclinical factors on the physical conditions of the nCoV patients were found significant in the research.

SARS-CoV-2 RT-PCR mediated identification Bangladesh multi-factorial analysis pre and post infection health conditions

References (59)

  1. 1 Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study [DOI]
  2. 2 Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR [DOI]
  3. 3 Correlation of Chest CT and RT-PCR Testing for Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases [DOI]
  4. 4 Hydroxychloroquine and azithromycin as a treatment of COVID-19: results of an open-label non-randomized clinical trial [DOI]
  5. 5 Mental Health and the Covid-19 Pandemic [DOI]
  6. 6 Pharmacologic Treatments for Coronavirus Disease 2019 (COVID-19) [DOI]
  7. 7 Age-dependent effects in the transmission and control of COVID-19 epidemics [DOI]
  8. 8 Association between short-term exposure to air pollution and COVID-19 infection: Evidence from China [DOI]
  9. 9 Physiological responses of the European cockle Cerastoderma edule (Bivalvia: Cardidae) as indicators of coastal lagoon pollution [DOI]
  10. 10 Clinical Features of 85 Fatal Cases of COVID-19 from Wuhan. A Retrospective Observational Study [DOI]
  11. 11 Renal Involvement and Early Prognosis in Patients with COVID-19 Pneumonia [DOI]
  12. 12 Smoking Is Associated With COVID-19 Progression: A Meta-analysis [DOI]
  13. 13 GIS-based spatial modeling of COVID-19 incidence rate in the continental United States [DOI]
  14. 14 First COVID-19 suicide case in Bangladesh due to fear of COVID-19 and xenophobia: Possible suicide prevention strategies [DOI]
  15. 15 Spatial analysis and GIS in the study of COVID-19. A review [DOI]
  16. 16 Evolving epidemiology and transmission dynamics of coronavirus disease 2019 outside Hubei province, China: a descriptive and modelling study [DOI]
  17. 17 Role of the chronic air pollution levels in the Covid-19 outbreak risk in Italy [DOI]
  18. 18 Enhancing immunity in viral infections, with special emphasis on COVID-19: A review [DOI]
  19. 19 Diagnostic Performance of CT and Reverse Transcriptase Polymerase Chain Reaction for Coronavirus Disease 2019: A Meta-Analysis [DOI]
  20. 20 Socio-Economic Impacts of COVID-19 on Household Consumption and Poverty [DOI]
  21. 21 Identification of Coronavirus Isolated from a Patient in Korea with COVID-19 [DOI]
  22. 22 Coronavirus Disease 19 Infection Does Not Result in Acute Kidney Injury: An Analysis of 116 Hospitalized Patients from Wuhan, China [DOI]
  23. 23 The COVID-19 Pandemic: Effects on Low- and Middle-Income Countries [DOI]
  24. 24 Active smoking is not associated with severity of coronavirus disease 2019 (COVID-19) [DOI]
  25. 25 Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus [DOI]
  26. 26 Initial psychological impact of COVID-19 and its correlates in Indian Community: An online (FEEL-COVID) survey [DOI]
  27. 27 COVID-19 and Smoking [DOI]
  28. 28 Probiotic Lactobacillus Strains Stimulate the Inflammatory Response and Activate Human Macrophages [DOI]
  29. 29 Challenges of COVID-19 in children in low- and middle-income countries [DOI]
  30. 30 Probiotics in respiratory virus infections [DOI]
  31. 31 Follow-up studies in COVID-19 recovered patients - is it mandatory? [DOI]
  32. 32 Laboratory Testing Methods for Novel Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) [DOI]
  33. 33 Smoking, SARS-CoV-2 and COVID-19: A review of reviews considering implications for public health policy and practice [DOI]
  34. 34 Prevalence of Comorbidities in COVID-19 Patients: A Systematic Review and Meta-Analysis. [DOI]
  35. 35 Thromboembolic events and Covid-19 [DOI]
  36. 36 Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens [DOI]
  37. 37 Are healthy smokers really healthy? [DOI]
  38. 38 Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples [DOI]
  39. 39 The clustering and transmission dynamics of pandemic influenza A (H1N1) 2009 cases in Hong Kong [DOI]
  40. 40 COVID-19, acute respiratory distress syndrome (ARDS), and hyperbaric oxygen therapy (HBOT): what is the link? [DOI]
  41. 41 Mental status of patients with chronic insomnia in China during COVID-19 epidemic [DOI]
  42. 42 Nocturnal oxygen therapy as an option for early COVID-19 [DOI]
  43. 43 In ovo inoculation of duck embryos with different strains of Bacillus cereus to analyse their synergistic post‐hatch anti‐allergic potentialities [DOI]
  44. 44 Aerosolization of COVID-19 and Contamination Risks During Respiratory Treatments.
  45. 45 Evaluation of Potential Probiotic Characteristics of Isolated Lactic Acid Bacteria from Goat Milk [DOI]
  46. 46 Manifestation of GIS Tools for Spatial Pattern Distribution Analysis of Dengue Fever Epidemic in the City of Subang Jaya, Malaysia [DOI]
  47. 47 Opsonin and its mechanism of action in secondary immune response [DOI]
  48. 48 Use of reverse transcriptase polymerase chain reaction to monitor expression of intronless genes.
  49. 49 Coronavirus COV-19/SARS-CoV-2 affects women less than men: clinical response to viral infection. [DOI]
  50. 50 COVID-19 attack rate increases with city size [DOI]
  51. 51 Rapid spread of the SARS-CoV-2 Delta variant in some French regions, June 2021 [DOI]
  52. 52 Preexisting Comorbidities Predicting COVID-19 and Mortality in the UK Biobank Community Cohort [DOI]
  53. 53 Sleep Pattern Changes in Nursing Students during the COVID-19 Lockdown [DOI]
  54. 54 Anticoagulant treatment in COVID-19: a narrative review [DOI]
  55. 55 Emerging pharmacological therapies for ARDS: COVID-19 and beyond [DOI]
  56. 56 Anticoagulant interventions in hospitalized patients with COVID‐19: A scoping review of randomized controlled trials and call for international collaboration [DOI]
  57. 57 Clinical Findings of COVID-19 Patients Admitted to Intensive Care Units in Guangdong Province, China: A Multicenter, Retrospective, Observational Study [DOI]
  58. 58 Estimating the number of Korean workers at high risk of COVID-19 infection [DOI]
  59. 59 Early Epidemiological analysis of CoVID-19: First report from South of Iran [DOI]

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