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Research Article Open access CC BY 4.0

Recovery of Biomass Energy on the Farm: Identification of Cellulolytic Bacteria in Agricultural Residues for On-Site Bioethanol Production

F. Laframboise, F. Meddeb-Mouelhi, S. Barnabé, M. Beauregard

Biotechnology Journal International · pp. 1–8 · Published 13 Aug 2016

10.9734/BBJ/2016/27841

Abstract

Farms are significant sources of unused biomass, conversion of which into energy would contribute to decreasing the environmental footprint associated with farming activities. A promising alternative for energy conversion involves bioethanol production. Bioethanol can be fermented from simple sugars that in turn must be extracted from biomass. To this end cocktails of enzymes may be used to deconstruct lignocellulosic biomass, but their cost and efficiency are often prohibitive. One could circumvent these drawbacks by finding locally-established, well adapted bacteria that produce enzymes with relevant specificities.  Here we identified such bacteria and compared their ability to hydrolyse cellulose from agricultural and industrial biomass residues. By collecting environmental samples at a local farm we identified 54 strains, of which 12 exhibited cellulolytic activity. Based on 16S rDNA sequence analyses, we found that these strains were relatives of Bacillus aryabhattai, B. cereus, B. licheniformis, B. pseudomycoides and B. thuringiensis species. This article reveals the first experimental evidence of cellulase activity from B. aryabhattai, confirming earlier predictions. The abilities of these strains to produce simple sugars from carboxymethyl cellulose, treated maize biomass, and papermaking primary sludge were investigated. B. licheniformis and B. thuringiensis related strains both showed high extracellular cellulase activity and sugar production when grown on treated maize.  This study suggests that local microbial biodiversity should be considered when developing enzymatic strategies for exploitation of farm residues.

Bioethanol biomass cellulase cellulose Bacillus aryabhattai Bacillus licheniformis Bacillus thuringiensis

References (19)

  1. 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  2. 2 Measurement of cellulase activities [DOI]
  3. 3 National Renewable Energy Laboratory [DOI]
  4. 4 Bioethanol production from agricultural wastes: An overview [DOI]
  5. 5 Manual of Environmental Microbiology [DOI]
  6. 6 Outlook for cellulase improvement: Screening and selection strategies [DOI]
  7. 7 A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram’s Iodine [DOI]
  8. 8 Microbial diversity of cellulose hydrolysis [DOI]
  9. 9 the Phylogenetic Handbook : A Practical Approach to DNA and protein phylogeny
  10. 10 Environmental impacts of a lignocellulose feedstock biorefinery system: An assessment [DOI]
  11. 11 Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere [DOI]
  12. 12 Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass [DOI]
  13. 13 The Phylogeny of Bacillus cereus sensu lato [DOI]
  14. 14 Isolation of Cellulase-Producing Bacteria and Characterization of the Cellulase from the Isolated Bacterium Cellulomonas Sp. YJ5 [DOI]
  15. 15 The Impact of Ethanol Use on Food Prices and Greenhouse-Gas Emissions
  16. 16 The application of viable count procedures for measuring viable cells in the various marine environments [DOI]
  17. 17 Draft genome of bagasse-degrading bacteria Bacillus aryabhattai GZ03 from deep sea water [DOI]
  18. 18 Characterization of Bacteria Community Isolated from Wood Decay [DOI]
  19. 19 The phylogenetic handbook: A practical approach to DNA and protein phylogeny [DOI]

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