Quantitative Risk Assessment for Salmonella in Lettuce (Lactuca sativa) Consumed in Benin, West Africa
D. Sylvain Dabadé, Vioutou E. C. Coffi, Paulin Azokpota
Microbiology Research Journal International · pp. 1–15 · Published 30 Nov 2022
10.9734/mrji/2022/v32i91341Abstract
The present study aims at quantitatively assessing the risk associated with Salmonella in lettuce (Lactuca sativa) consumed in Benin (West Africa). To that end, a survey was conducted involving 550 respondents to determine the conditions under which the product is handled along the supply chain and its consumption pattern. The prevalence and concentration of Salmonella in lettuce were collected from the literature. The consumption data and the data on Salmonella concentrations in lettuce were combined to estimate the exposure to Salmonella using a probabilistic risk assessment method. The @Risk software package (Palisade USA) was used to run Monte Carlo simulations with 10,000 iterations. Three dose-response models were used to assess the risk of salmonellosis. Different scenarios were tested to identify factors that could influence the risk of salmonellosis. The results showed that lettuce is exposed to temperature abuse under inappropriate hygienic conditions. In 90% of the cases, the exposure to Salmonella was between 3 and 7 log CFU/serving. The risk of salmonellosis per serving varied from 7.7% to 95% depending on the dose-response used with the scenario taking into account the current handling conditions of the lettuce. In contrast, when considering the scenario where the cold chain is respected along the supply chain, the risk of salmonellosis varied from 0% to 3.3% depending on the dose response used. The study highlights the importance of the cold chain, good agricultural practices and good hygienic practices to reduce exposure to Salmonella through the consumption of lettuce and thus the risk of salmonellosis.
References (45)
- 1 A Review of Human Salmonellosis: I. Infective Dose [DOI]
- 2 Irrigation Water as Source of Foodborne Pathogens on Fruit and Vegetables [DOI]
- 3 Review of Salmonella detection and identification methods: Aspects of rapid emergency response and food safety [DOI]
- 4 Fate of Salmonella enterica Serovar Typhimurium on Carrots and Radishes Grown in Fields Treated with Contaminated Manure Composts or Irrigation Water [DOI]
- 5 Quantitative Microbial Risk Assessment Models for Consumption of Raw Vegetables Irrigated with Reclaimed Water [DOI]
- 6 Foodborne Outbreaks in Canada Linked to Produce: 2001 through 2009 [DOI]
- 7 A Large Escherichia coli O157 Outbreak in Sweden Associated with Locally Produced Lettuce [DOI]
- 8 Irrigated urban vegetable production in Ghana: microbiological contamination in farms and markets and associated consumer risk groups [DOI]
- 9 A national outbreak of multi-resistant Salmonella enterica serovar Typhimurium definitive phage type (DT) 104 associated with consumption of lettuce [DOI]
- 10 Outbreak of Salmonella Thompson Infections Linked to Imported Rucola Lettuce [DOI]
- 11 Prevalence and trends of bacterial contamination in fresh fruits and vegetables sold at retail in Canada [DOI]
- 12 Estimation of the relative contribution of different food and animal sources to human Salmonella infections in the European Union [DOI]
- 13 Salmonella spp. and Escherichia coli O157:H7 prevalence and levels on lettuce: A systematic review and meta-analysis [DOI]
- 14 New insights into cross-contamination of fresh-produce [DOI]
- 15 Efficacy of Antimicrobial Agents in Lettuce Leaf Processing Water for Control of Escherichia coli O157:H7 [DOI]
- 16 Quantitative Microbial Risk Assessment for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in Leafy Green Vegetables Consumed at Salad Bars [DOI]
- 17 Cross-Contamination of Lettuce with Escherichia coli O157:H7 [DOI]
- 18 IRRIGATION WATER AS A POTENTIAL PREHARVEST SOURCE OF BACTERIAL CONTAMINATION OF VEGETABLES [DOI]
- 19 Prevalence and diversity of Salmonella enterica in water, fish and lettuce in Ouagadougou, Burkina Faso [DOI]
- 20 Validity of photographs for food portion estimation in a rural West African setting [DOI]
- 21 Zero Risk Does Not Exist: Lessons Learned from Microbial Risk Assessment Related to Use of Water and Safety of Fresh Produce [DOI]
- 22 Risk assessment and risk management for safe foods: Assessment needs inclusion of variability and uncertainty, management needs discrete decisions [DOI]
- 23 Moving towards a risk-based food safety management [DOI]
- 24 Monte Carlo Simulations Assessing the Risk of Salmonellosis from Consumption of Almonds [DOI]
- 25 Foodborne pathogens and their risk exposure factors associated with farm vegetables in Rwanda [DOI]
- 26 Multiple outbreaks ofSalmonella braenderupassociated with consumption of iceberg lettuce [DOI]
- 27 Sextuplex PCR combined with immunomagnetic separation and PMA treatment for rapid detection and specific identification of viable Salmonella spp., Salmonella enterica serovars Paratyphi B, Salmonella Typhimurium, and Salmonella Enteritidis in raw meat [DOI]
- 28 Reduction of microbial counts during kitchen scale washing and sanitization of salad vegetables [DOI]
- 29 Diversity and antimicrobial drug resistance of non-typhoid Salmonella serotypes isolated in lettuce, irrigation water and clinical samples in Burkina Faso [DOI]
- 30 Risk assessment of Salmonella in Danish meatballs produced in the catering sector [DOI]
- 31 Modelling the combined effect of chlorine, benzyl isothiocyanate, exposure time and cut size on the reduction of Salmonella in fresh-cut lettuce during washing process [DOI]
- 32 Real-time PCR detection of Salmonella species using a novel target: the outer membrane porin F gene (ompF) [DOI]
- 33 Salmonellaimportance and current status of detection and surveillance methods [DOI]
- 34 Salmonella surveillance on fresh produce in retail in Turkey [DOI]
- 35 Effect of irrigation water on the incidence of Salmonella spp. on lettuces produced by urban agriculture and sold on the markets in Dakar, Senegal [DOI]
- 36 Atlas of West African urban food systems: examples from Ghana and Burkina Faso [DOI]
- 37 Quality Perceptions of Stakeholders in Beninese Export-Oriented Shrimp Chain [DOI]
- 38 Potential Links between Irrigation Water Microbiological Quality and Fresh Vegetables Quality in Upper East Region of Ghana Subsistence Farming [DOI]
- 39 Prevalence and antibioresistance of Escherichia coli and Salmonella isolated from lettuce and irrigation water in Ouagadougou, Burkina Faso [DOI]
- 40 Assessment of the Risk of Microbial Contamination of an Urban Crop in the City of Daloa (Côte d'Ivoire): Case of Lettuce (Lactuca sativa L.) [DOI]
- 41 Agricultural practices in Brazilian organic farms and microbiological characteristics of samples collected along the production chain [DOI]
- 42 SALMONELLOSIS AT RURAL AND URBAN CLINICS IN BANGLADESH [DOI]
- 43 Handling Practices and Quality Attributes Along the Supply Chain of gboma (Solanum Macrocarpon): A Leafy Vegetable in Southern Benin [DOI]
- 44 International Water Management Institute (IWMI) [DOI]
- 45 Integrated pest management in vegetable production: a guide for extension workers in West Africa
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