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Molecular Detection of Plasmid-Mediated Quinolone Resistance Genes (qnrA and aac(6′)-Ib-cr) in Drug Resistant Escherichia coli, Sudan

Mohamed Tageldin, Adel Mergani Babier, Omer Abu Elhasan, Mirghani Yousif, Seitelbanat Yasin Ahemir, Hajir Mohammed Hussien, Sanaa Mohammed Yousif, Salma Omer Ibrahim, Hadeel Omer Mirgani, Hind Elhaj Mohamed, Tarig A Gamar, Elhadi Abdalla Ahmed

Microbiology Research Journal International · pp. 14–21 · Published 19 Jun 2023

10.9734/mrji/2023/v33i41375

Abstract

Background: The quinolone group, a synthetic antimicrobial, is widely used worldwide to treat many diseases, including those caused by Gram-negative bacteria. Escherichia coli and others are among the bacteria that produce quinolone resistance genes (qnr) such as qnrA and aac(6′)-Ib-cr.  Objective: The present study aimed to the isolate Escherichia coli from patients attending some Hospitals in Wad Medani city, identification of drug resistance patterns and detection of the frequency of quinolones resistance genes; qnrA and aac(6′)-Ib-cr among isolated strains. Methods: A cross-sectional descriptive, hospital-based study included 119 Escherichia coli strains was conducted. A designed questionnaire used for demographic data collection and the attitude toward antimicrobials usage. Clinical specimens were processed for aerobic bacterial isolation and identification. Antimicrobial sensitivity performed by Kirby Bauer disc diffusion technique according to the CLSI guidelines. Presence of qnrA and aac(6′)-Ib-cr genes was assessed by multiplex PCR. Results: Most strains of Escherichia coli originated from urine 53.8% (64/119) and wounds 42.9% (51/119) specimens. Meropenem had the best effect against tested strains with susceptibility of 85% (101/119). Multiplex PCR assay, using specific primers, demonstrated that 41.2% (49/119) and 37.8% (45/119) of isolated Escherichia coli possessed qnrA and aac(6′)-Ib-cr genes respectively. Conclusion: The high rate of qnrA and aac (6)-Ib-cr genes among Escherichia coli necessitate the usage of molecular tools in detecting the genetic determinants of drug resistance microorganisms in countries such as Sudan.

Escherichia coli quinolone resistanc qnrA aac(6′)-Ib-cr multiplex-PCR Sudan

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Cited by 1

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Mohammed Fadhil AboKsour, M. Al–Marjani, A. Rheima · Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ) · 2024

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