Mechanism of Anticonvulsant Effects of Ethanol Leaf Extract and Fractions of Milicia excelsa (Moraceae) in Mice
Lateef Abiola Akinpelu, Moses Atanda Akanmu, Efere Martins Obuotor
Journal of Pharmaceutical Research International · pp. 1–11 · Published 8 Sep 2018
10.9734/JPRI/2018/42430Abstract
Aims: This study investigated the anticonvulsant potential of ethanol leaf extract and fractions of Milicia excelsa (Moraceae). Study Design: This study used experimental animal models predictive of human convulsion in mice. Place and Duration of Study: Department of Pharmacology, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria, between January 2014 and February 2015. Methodology: The anticonvulsant effect of ethanol leaf extract (EME), n-hexane (HF), ethyl acetate (EAF), n-butanol (BF) and aqueous (AF) fractions of the extract was evaluated using picrotoxin-, pentylenetetrazole-, and strychnine-induced convulsion models. The neural mechanism of anticonvulsant effect of the most active fraction (AF) was also investigated using flumazenil (3 mg/kg, i.p.), cyproheptadine (4 mg/kg, i.p.) and L-NG-Nitroarginine (10 mg/kg, i.p.) in picrotoxin-induced convulsion model. Results: EME and AF significantly (P < .05) delayed the onset of clonic and tonic convulsions and prolonged death latency with varying degrees of protection in picrotoxin-, and pentylenetetrazole-induced convulsion models. EME, EAF and AF significantly (P < .05) pronlonged the onset of clonic convulsion in strychnine-induced convulsion in mice. Flumazenil, cyproheptadine and L-NG-Nitroarginine abolished the anticonvulsant effect of AF suggesting the involvement of GABAergic, serotonergic and nitergic pathways. Conclusion: This study concludes that Milicia excelsa leaf contains biologically active anticonvulsant principles, thus lending pharmacological credence to the suggested traditional use. Further study may be undertaken to isolate and elucidate the chemical structure of the biologically active ingredient(s) responsible for the observed anticonvulsant effect.
References (35)
- 1 Antiepileptic Drug Mechanisms of Action [DOI]
- 2 Maternal Use of Antiepileptic Drugs and the Risk of Major Congenital Malformations: A Joint European Prospective Study of Human Teratogenesis Associated with Maternal Epilepsy [DOI]
- 3 Development of new antiepileptic drugs: challenges, incentives, and recent advances [DOI]
- 4 AN ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS AMONG THE BAKA PYGMIES IN THE DJA BIOSPHERE RESERVE, CAMEROON [DOI]
- 5 The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine.
- 6 Cognitive side-effects of chronic antiepileptic drug treatment: A review of 25 years of research [DOI]
- 7 5-HT2 Receptor Regulation of Extracellular GABA Levels in the Prefrontal Cortex [DOI]
- 8 New antiepileptic drugs that are second generation to existing antiepileptic drugs [DOI]
- 9 Anticonvulsant effects of aerial parts of Passiflora incarnata extract in mice: involvement of benzodiazepine and opioid receptors [DOI]
- 10 Anticonvulsant Activity of Carissa carandas Linn. Root Extract in Experimental Mice [DOI]
- 11 A review of medicinal plants showing anticonvulsant activity [DOI]
- 12 Review on phytotherapy in epilepsy [DOI]
- 13 Ethnomedical Study and Iron Content of Some Medicinal Herbs Used in Traditional Medicine in Cote D’Ivoire for the Treatment of Anaemia [DOI]
- 14 An Ethnobotanical and Floristical Study of Medicinal Plants Among the Baka Pygmies in the Periphery of the Ipassa- Biosphere Reserve, Gabon [DOI]
- 15 Ursolic acid plays a role in Nepeta sibthorpii Bentham CNS depressing effects [DOI]
- 16 Ethno-Medicinal Plants And Methods Used By Gwandara Tribe Of Sabo Wuse In Niger State, Nigeria, To Treat Mental Illness [DOI]
- 17 Ethnobotanical study of commonly used medicinal plants of the Takamanda Rainforest South West, Cameroon [DOI]
- 18 Anticonvulsant Effect of Drosera burmannii Vahl
- 19 Evaluation and Treatment of Seizures [DOI]
- 20 Antagonism of caffeine-induced seizures in mice by Ro15-1788 [DOI]
- 21 Anticonvulsant Effect of Flutamide on Seizures Induced by Pentylenetetrazole: Involvement of Benzodiazepine Receptors [DOI]
- 22 The promise of new antiepileptic drugs [DOI]
- 23 Cognitive and neurodevelopmental effects of antiepileptic drugs [DOI]
- 24 Plants and Plant Products with Potential Anticonvulsant Activity – A Review [DOI]
- 25 Anticonvulsant effect of FS‐1 subfraction isolated from roots of Delphinim denudatum on hippocampal pyramidal neurons [DOI]
- 26 Anticonvulsant and free radical scavenging activity of Hybanthus enneaspermus: A preliminary screening
- 27 Anticonvulsive Effect of Seed Extract of Caesalpinia bonducella (Roxb.)
- 28 Psychopharmacological Potentials of Methanol Leaf Extract of Securinega virosa Roxb (Ex Willd) Baill. in Mice [DOI]
- 29 Assessment of the Anticonvulsant Potency of Ursolic Acid in Seizure Threshold Tests in Mice [DOI]
- 30 Anticonvulsant, Anxiolytic and Hypnotic effects of Aqueous Bulb Extract of Crinum glaucum A. Chev (Amaryllidaceae): Role of GABAergic and Nitrergic Systems [DOI]
- 31 Preliminary Studies on Some Medicinal Plants in Girei, Adamawa State of Nigeria [DOI]
- 32 Excelsoside: A New Benzylic Diglycoside from the Leaves of Milicia excelsa [DOI]
- 33 BTS 72664 – A Novel CNS Drug With Potential Anticonvulsant, Neuroprotective, and Antimigraine Properties [DOI]
- 34 Anticonvulsant Activity of Schumanniophyton magnificum Roots Extracts in Mice [DOI]
- 35 Anticonvulsant and Depressant Activity of Methanol Leaf Extract of Croton zambesicus [DOI]
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