Influence of Abiotic Factors on Anti-reproductive Activity of Bait-containing Papain in Lymnaea acuminata
Arun Kumar Srivastava, D. K. Singh, Vinay Kumar Singh
Annual Research & Review in Biology · pp. 223–237 · Published 5 Oct 2013
10.9734/ARRB/2014/4163Abstract
Aims: To investigate the anti-reproductive action of papain against Lymnaea acuminata with respect to abiotic factors. Snail Lymnaea acuminata is the intermediate host of Fasciola species, which causes endemic fasciolosis in cattle and human population. Fasciolosis is a disease of ruminants worldwide which shows most widespread distribution in comparison to other vector-born parasitic disease. Study Design: Baits were prepared from starch or serine (20Mm) in 2% agar-agar solution with papain the active component of Carica papaya (40% and 80% of 24h LC50). Each regimen of 5 liter water was kept in six aquaria separately, containing 20 snails in each aquarium. Bait containing papain was added in each aquarium except control. Control bait was without papain. After every 24 h, up to 96 h, the total number of eggs laid by the snails was counted in each aquarium. At every 24h spawns were observed for hatching and survival of embryos. Temperature, pH, dissolved oxygen and dissolved free carbon dioxide of different regimen of water were measured, simultaneously. After 96h, the ovotestis and/ or nervous tissue were dissected out and protein [22], free amino acids [23], nucleic acids [24] and enzyme AChE [25,26] activity were then measured. Place and Duration of Study: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur – 273009, U.P., India.The present study was carried outinbetween November- 2011 to October- 2012. Results: Feeding of baits containing papain with starch and serine (40% and 80% of 24h LC50) caused a significant reduction in fecundity, hatchability and survival of young snails. Maximum (1036 eggs/20 snails) and minimum (175 eggs/ 20 snails) fecundity were observed in June-2012 and February-2012, respectively. There was significant (P =.05) positive correlation in between the sublethal concentration of baits containing papain (starch/ serine) and variation of temp/pH/CO2 of water in each month of the year2011-12. Protein, amino acid, DNA and RNA in the ovotestis of L. acuminata were significantly decreased when they were fed to bait containing 80% of 24h LC50 of papain + serine in different months of 2011 and 2012. Maximum reduction in protein (26.31 % of control), amino acid (28.83% of control), DNA (42.34% of control) and RNA (34.10% of control) level were measured in the ovotestis of L. acuminata fed to bait containing 80% of 24h LC50 of papain + serine. Feeding of bait containing 80% of 24h LC50 of papain + starch caused maximum reduction in the level of protein (28.97% of control), amino acid (56.77% of control), DNA (42.05% of control) and RNA (29.31% of control) in the ovotestis of L. acuminata. There was a significant (P=.05) positive correlation between the fecundity in different months and the corresponding AChE activity in the nervous tissue of the snail. Feeding of bait containing 80% of 24h LC50 of papain + serine caused maximum inhibition in AChE activity (41.71% of control) was observed in snail exposed to in the nervous tissue of L. acuminata in December2011. Feeding of bait containing 80% of 24h LC50 of papain + starch caused maximum inhibition in AChE activity (31.92% of control) in the nervous tissue of L. acuminata in June 2012. Conclusion: Papain altered the reproductive capacity of snails. The anti-reproductive action against L. acuminata significantly altered with respect to the change in the abiotic factors in different months of the year Nov-2011toOct-2012.
References (26)
- 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
- 2 Climate variations and the physiological basis of temperature dependent biogeography: systemic to molecular hierarchy of thermal tolerance in animals [DOI]
- 3 Fascioliasis and other plant-borne trematode zoonoses [DOI]
- 4 Toxicology of Insecticides [DOI]
- 5 Chapter 2 Fasciola, Lymnaeids and Human Fascioliasis, with a Global Overview on Disease Transmission, Epidemiology, Evolutionary Genetics, Molecular Epidemiology and Control [DOI]
- 6 Introduction to Biostatistics. [DOI]
- 7 Regulation of intracellular pH in eukaryotic cells [DOI]
- 8 POLO: A New Computer Program for Probit Analysis [DOI]
- 9 Toxicology of Insecticides [DOI]
- 10 "Fasciola gigantica": Epidemiology, control, immunology and molecular biology
- 11 Pest slugs and snails. Biology and control.
- 12 Molluscicides of Plant Origin [DOI]
- 13 The energetic cost of protein synthesis in isolated hepatocytes of rainbow trout (Oncorhynchus mykiss) [DOI]
- 14 Harmful gastropods and their control [DOI]
- 15 Molluscicidal activity of Carica papaya and Areca catechu against the freshwater snail Lymnaea acuminata [DOI]
- 16 Human fascioliasis in some countries of the Eastern Mediterranean Region [DOI]
- 17 Behavioural responses of the snail Lymnaea acuminata to carbohydrates in snail-attractant pellets [DOI]
- 18 Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species [DOI]
- 19 Pestiferous Gastropods and Their Control
- 20 The effect of abiotic factors on certain biochemical changes in ovotestis of snail Lymnaea acuminata: intermediate host of trematode diseases.
- 21 Folin Colorimetric Procedure for Amino Acids [DOI]
- 22 Comparative study of cholinesterase in two snails Pila globosa and Lymnaea acuminata.
- 23 Comparative Endocrinology of the Invertebrates [DOI]
- 24 Seasonal Fluctuations in the Secretory Response of Neuroendocrine Cells ofAplysia californicato Inhibitors of Protein Kinase A and Protein Kinase C [DOI]
- 25 Chapter 32 The anti-gonadotropic neuropeptide schistosomin interferes with peripheral and central neuroendocrine mechanisms involved in the regulation of reproduction and growth in the schistosome-infected snail Lymnaea stagnalis [DOI]
- 26 Introduction to Biostatistics [DOI]
Cited by 12
A. Srivastava, V. Singh · 2018
Navneet Kumar, D. Singh, V. Singh · Scientifica · 2016
Dinesh Singh, Vinay Singh, Raghubir Singh · Fasciolosis: Causes, Challenges and Controls · 2021
A. Srivastava, V. Singh · 2018
A. Sriv, V. Sing · 2015
A. Srivastava, A. Tripathi, P. Srivastava · 2014
Showing 6 of 12 known citations — external sources report more than can currently be individually listed.
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