In vivo Trypanocidal Activity of Ethanolic Crude Extract and Phytochemical Fractions of Garcinia kola Seeds
Annual Research & Review in Biology · pp. 212–222 · Published 5 Oct 2013
10.9734/ARRB/2014/5716Abstract
Aim: To determine the trypanocidal activity of the crude ethanolic extract and phytochemical fractions of Garcinia kola seeds in Trypanosoma brucei brucei infected rats. Study Design: Trypanosoma brucei brucei infected rats were treated with the ethanolic crude extract, alkaloid, flavonoid, saponin and cardiac glycoside fractions of Garcinia kola seeds. Parasitaemia and Packed cell volume (PCV) were measured. Place and Duration of Study: Department of Biochemistry, University of Jos, Jos, Nigeria between June and October 2011. Methodology: 11 groups of four rats each were used. Group A rats were infected but not treated. Groups B, C, D, E and F were infected but treated with 50mg/kg crude extract, alkaloid, flavonoid, saponin and cardiac glycoside fractions respectively, while groups G, H, I, J and K were infected but treated with 100mg/kg of the extracts respectively. Parasitaemia levels of rats were observed daily under Light microscope at x100 magnification. PCV values were determined on days 4 and 8 using microhaematocrit centrifuge at 10,000xg for 5 min. Results: At 50mg/kg the crude extract brought about a 37.50% reduction in the parasitaemia of infected rats, the alkaloid fraction 76.70%, flavonoid fraction 53.45% and saponin fraction 76.70%. At 100mg/kg the crude extract and the alkaloid, flavonoid and saponin fractions reduced parasitaemia of infected rats by 84.50%, 92.25%, 61.20% and 68.98% respectively. Treatment with the cardiac glycoside fraction showed no improvement in parasitaemia. Significant increases (P=.05) in PCV values were observed in all the treated groups on days 4 and 8 when compared with the untreated ones. Conclusion: Available results from this research shows that ethanolic crude extract of Garcinia kola seeds and its alkaloid, flavonoid and saponin fractions at 50 and 100mg/kg demonstrated activities against Trypanosoma brucei brucei in rats.
References (21)
- 1 Flavonoids as Antioxidants [DOI]
- 2 Control of Neglected Tropical Diseases [DOI]
- 3 Saponins as cytotoxic agents: a review [DOI]
- 4 Herbal Extracts and Phytochemicals: Plant Secondary Metabolites and the Enhancement of Human Brain function [DOI]
- 5 Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron—A Garcinia kola seed extract [DOI]
- 6 African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents [DOI]
- 7 Antimalarial activity of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo [DOI]
- 8 Drugs for neglected diseases initiative [DOI]
- 9 Natural Products Active Against African Trypanosomes: A Step Towards New Drugs [DOI]
- 10 Microbiological exploitation of cardiac glycosides and alkaloids from Garcinia kola, Borreria ocymoides, Kola nitida and Citrus aurantifolia [DOI]
- 11 Powerful antioxidative agents based on garcinoic acid from Garcinia kola [DOI]
- 12 In Vitro Effect of Alkaloids on Bloodstream Forms of Trypanosoma brucei and T. congolense [DOI]
- 13 Protective effects of garcinia kola seed extract against paracetamol-induced hepatotoxicity in rats [DOI]
- 14 Chemotherapeutic effects ofAnnona senegalensisinTrypanosoma brucei brucei [DOI]
- 15 Capillary Electrophoresis Determination of Biflavanones from Garcinia kola in Three Traditional African Medicinal Formulations [DOI]
- 16 Evaluation of Medicinal Plants from Nupeland for Their in vivo Antitrypanosomal Activity [DOI]
- 17 Lavender lactone and other volatile constituents of the oleoresin from seeds ofGarcinia kola Heckel [DOI]
- 18 Inhibitory effect of Garcinia kola on lipid peroxidation in rat liver homogenate.
- 19 In vitro Studies on the Trypanocidal activities of various Phytochemical fractions obtained from Garcinia kola seed [DOI]
- 20 Serum Iron and Nitric Oxide Production in Trypanosoma brucei Infected Rats Treated with Tetracycline
- 21 Capillary Electrophoresis Determination of Biflavanones from Garcinia Kola in Three Traditional African Medicinal Formulations [DOI]
Cited by 10
Abdulrahman.Mahmud Dogara, S. Hamad, H. Hama · Advances in pharmacological and pharmaceutical sciences · 2022
N. Nwodo, A. Ibezim, F. Ntie-Kang · Molecules · 2015
A. B. David, A. Inuwa, S. Malgwi · 2020
N. Mathew, P. Negi · Discovery and Development of Therapeutics from Natural Products Against Neglected Tropical Diseases · 2019
A. Richard, W. M. Peter, Efe M. Omwirhiren · 2018
Mohamed Dkhil, Saeed El-Ashram, Rewaida Abdel-Gaber · Infectious Diseases · 2024
Lawal, S. M., Inabo, H. I., Ella, E. E. · UMYU Journal of Microbiology Research (UJMR) · 2024
Showing 7 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Nutrient Analysis and Antimicrobial Activities of the Leaves and Fruit Pulp Extracts of Tetrapluera tetraptera on Clinical Bacteria Isolates — shares topic coverage
- Antibacterial and Phytochemical Activity of Gliricidia sepium against Poultry Pathogens — shares topic coverage
- Antibacterial Screening of Two Indigenous Nigerian Spices; Piper guineense and Xylopia aethiopica — shares topic coverage
- In vitro Antibacterial Activity of the Extracts of Peperomia pellucida (L) — shares topic coverage
- Phytochemical and Antibacterial Investigation of Leaf Extracts of Vernonia amygdalina — shares topic coverage
Article metrics
Real usage data collected on this platform.
78
Page views
0
PDF downloads
35
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.