Skip to content
Research Article Open access CC BY 3.0

Identification of Genes Putatively Involved in the Biosynthesis of Antitubercular Peptide in Streptomyces ribosidificus NRRL B-11466

Khaled M. Aboshanab, Nisreen M. Okba, Tarek S. El-banna, Ahmed A. Abd El-Aziz

Biotechnology Journal International · pp. 40–50 · Published 26 Oct 2013

10.9734/BBJ/2014/5210

Abstract

Aims: To determine the potential antitubercular activity of Streptomyces ribosidificus NRRL B-11466 both on genotypic and phenotypic levels.  Methodology: Standard methods and software programs were used for nucleotide/protein sequence analysis and phenotypic detection of antitubercular activity. Results: Analysis of the submitted DNA segment (accession code = AJ744850) harbouring the ribostamycin biosynthetic gene cluster showed that the respective gene cluster was flanked in the upstream region by three open reading frames (ORFs), encoding putative type II thioesterase (SribL03.14c) and two nonribosomal peptide synthases (SribL03.14c and SribL03.14c). These ORFs were of high amino acid similarities (about 80%) to those located in the viomycin and related antibiotic biosynthetic gene clusters. A DNA segment harbouring three ORFs, putatively involved in capreomycidine biosynthesis was submitted into the GenBank database under the accession code HQ327309. Comparative analysis of the respective DNA segment with viomycin and related antibiotic biosynthetic gene clusters showed: firstly, location of the respective DNA segment in the neighbourhood and upstream to the ribostamycine biosynthetic gene cluster; secondly, conservation of six ORFs: SriC (putative L-arginine hydroxylase); SriD (putative L-capreomycidine synthase), SriE (putative permease) located on our submitted DNA fragment; and SribL03.14c, SribL03.15c, SribL03.16c located on the DNA fragment harboring ribostamycin biosynthetic gene cluster, among the tested biosynthetic gene clusters. Phenotypically, S. ribosodificus inhibited growth of Mycobacterium smegmatis ATCC 19420 and Mycobacterium phlei ATCC 11758. Conclusion: Streptomyces ribosidificus NRRL B-11466 produces antimycobacterial agents and this was confirmed genotypically via detection of 6 ORFs with high amino acid similarities (about 80%) to those located in the viomycin and related antibiotic biosynthetic gene clusters as well as phenotypically by determining its inhibitory activity against Mycobacterium smegmatis ATCC 19420. This is the first report about identification of genes putatively involved capreomycidine biosynthesis in Streptomyces ribosidificus NRRL B-11466.

Tuberactinomycins capreomycidine biosynthesis viomycin Streptomyces ribosidificus NRRL B-11466

References (19)

  1. 1 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice [DOI]
  2. 2 The staden sequence analysis package [DOI]
  3. 3 Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains [DOI]
  4. 4 FramePlot: a new implementation of the Frame analysis for predicting protein-coding regions in bacterial DNA with a high G+C content [DOI]
  5. 5 The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome [DOI]
  6. 6 Inhibition of the Self-cleavage Reaction of the Human Hepatitis Delta Virus Ribozyme by Antibiotics [DOI]
  7. 7 Deciphering Tuberactinomycin Biosynthesis: Isolation, Sequencing, and Annotation of the Viomycin Biosynthetic Gene Cluster [DOI]
  8. 8 Conversion of (2S)‐Arginine to (2S,3R)‐Capreomycidine by VioC and VioD from the Viomycin Biosynthetic Pathway of Streptomyces sp. Strain ATCC11861 [DOI]
  9. 9 Investigations into Viomycin Biosynthesis by Using Heterologous Production in Streptomyces lividans [DOI]
  10. 10 Bacterial protoplast fusion: Recombination in fused protoplasts of Streptomyces coelicolor [DOI]
  11. 11 Rapid identification and characterization of hammerhead-ribozyme inhibitors using fluorescence-based technology [DOI]
  12. 12 Identification and cloning of genes encoding viomycin biosynthesis from Streptomyces vinaceus and evidence for involvement of a rare oxygenase [DOI]
  13. 13 Streptothricin biosynthesis is catalyzed by enzymes related to nonribosomal peptide bond formation [DOI]
  14. 14 Biosynthesis of streptothricin F. 1. Observing the interaction of primary and secondary metabolism with [1,2-13C2]acetate [DOI]
  15. 15 Biosynthesis of viomycin. I. Origin of αβ-diaminopropionic acid and serine [DOI]
  16. 16 Cyclic homopentapeptides 1. Analogs of tuberactinomycins and capreomycin with activity against vancomycin-resistant enterococci and Pasteurella [DOI]
  17. 17 Biosynthesis of capreomycin. 1. Incorporation of arginine [DOI]
  18. 18 Cyclic homopentapeptides 3. Synthetic modifications to the capreomycins and tuberactinomycins: Compounds with activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci [DOI]
  19. 19 The Biochemistry and Genetics of Aminoglycoside Producers [DOI]

Cited by 0

No indexed citations yet.

Article metrics

Real usage data collected on this platform.

56

Page views

0

PDF downloads

23

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.