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Research Article Open access CC BY 4.0

Genetic, Protein and FTIR Spectroscopic Comparison of Anterior and Posterior Deciduous Dental Pulp for Subsequent Obtention of SHED

Gustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda, Raul Jacobo Delgado-Macuil, Marlon Rojas-Lopez, Rosbelia Ramos-Roldan, Olga Gonzalez Aguilar, Virginia Sanchez-Monroy

Annual Research & Review in Biology · pp. 1–11 · Published 3 Feb 2018

10.9734/ARRB/2018/38907

Abstract

Background: Stem cells from Human Exfoliated Deciduous teeth (SHED) were identified by Miura in 2003. SHED have been described as a suitable, accessible and potential source for regenerative medicine and therapeutic applications. However, the best group of deciduous teeth for the obtention of stem cells (SCs) has not been established. Therefore, this research aimed to determine the dental organs group from which SHED can be obtained with higher potentiality, considering their biomolecular features. Methodology: Deciduous teeth from 64 healthy children were collected and divided into two groups: anterior and posteriors. Dental pulp tissue was removed to determine their genetic, phenotypic, and spectroscopic profiles by RT-qPCR, immunofluorescence, and Fourier Transform Infrared (FTIR) spectroscopy respectively. Results: The results showed a higher gene (CD73 and NANOG) and protein (NANOG and SOX2) expression of mesenchymal and pluripotent markers in anterior SHED. CD146 gene expression between the two groups shows no statistical significant difference. Furthermore, the analysis of deciduous dental pulps by FTIR spectroscopy showed spectral bands related to biological samples, indicating the higher state of potentiality in anterior deciduous dental pulps. Conclusion: The deciduous dental pulp harbor a heterogenous population of SCs with different potentiality; however, the expression of multipotent and pluripotent markers was higher in the pulps from anterior deciduous teeth respect to posterior deciduous teeth. The storage and obtention of SHED from anterior teeth is more recommended respect to posterior teeth. However, it is necessary to analyze more stem cell markers and to study the differentiation capability of SHED.

Adult stem cells deciduous teeth dental pulp SHED regenerative medicine

References (25)

  1. 1 Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method [DOI]
  2. 2 Deficiencies in DNA damage repair limit the function of haematopoietic stem cells with age [DOI]
  3. 3 Concise Review: The Surface Markers and Identity of Human Mesenchymal Stem Cells [DOI]
  4. 4 Stem cells, ageing and the quest for immortality [DOI]
  5. 5 Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation [DOI]
  6. 6 Isolation and Characterization of a Population of Immature Dental Pulp Stem Cells Expressing OCT-4 and Other Embryonic Stem Cell Markers [DOI]
  7. 7 Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction [DOI]
  8. 8 Isolation and Characterization of Postnatal Stem Cells from Human Dental Tissues [DOI]
  9. 9 Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells [DOI]
  10. 10 Stem Cells from Deciduous Tooth Repair Mandibular Defect in Swine [DOI]
  11. 11 Reviewing and Updating the Major Molecular Markers for Stem Cells [DOI]
  12. 12 Stem Cells in Dental Pulp of Deciduous Teeth [DOI]
  13. 13 Banking Stem Cells from Human Exfoliated Deciduous Teeth (SHED): Saving for the Future [DOI]
  14. 14 Scaling-Up of Dental Pulp Stem Cells Isolated from Multiple Niches [DOI]
  15. 15 Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study [DOI]
  16. 16 Stem Cells, Their Niches and the Systemic Environment: An Aging Network [DOI]
  17. 17 Fourier Transform Infrared Microspectroscopy Identifies Symmetric PO2− Modifications as a Marker of the Putative Stem Cell Region of Human Intestinal Crypts [DOI]
  18. 18 The Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles [DOI]
  19. 19 CD73 Pathway Contributes to the Immunosuppressive Ability of Mesenchymal Stem Cells in Intraocular Autoimmune Responses [DOI]
  20. 20 Comparative Immunophenotypic Characteristics, Proliferative Features, and Osteogenic Differentiation of Stem Cells Isolated from Human Permanent and Deciduous Teeth with Bone Marrow [DOI]
  21. 21 FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells [DOI]
  22. 22 The International Stem Cell Initiative: toward benchmarks for human embryonic stem cell research [DOI]
  23. 23 Stem Cell Approaches for the Treatment of Renal Failure [DOI]
  24. 24 Human Fetal Mesenchymal Stem Cells [DOI]
  25. 25 From stem to stern [DOI]

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