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Research Article Open access CC BY 4.0

Effect of Arsenic Toxicity on Chlorophyll Content and Antioxidative Enzymes Activities in Rice Seedlings

Dinkar J. Gaikwad, Apurba Pal, Subhasis Mondal

International Journal of Environment and Climate Change · pp. 411–421 · Published 31 Dec 2020

10.9734/ijecc/2020/v10i1230318

Abstract

Aims: Arsenic (As) contamination in rice is at alarming level as majority of rice growing regions in India are As contaminated. Present investigation is designed to study the better understanding of the physiological and biochemical mechanisms in the amount of chlorophyll change and antioxidative enzymes activity under arsenic stress on rice variety IET-4786 (Shatabdi). Study Design: Completely Randomized Design. Place and Duration of Study: The experiment was carried out in the departmental laboratory of Plant Physiology, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Mohanpur, Nadia,West Bengal during the year 2017-18. Methodology: Two classes of inorganic arsenic- As(V) in the form of Sodium arsenate (Na2HAsO4.7H2O, M. W. = 321.01) and As(III) in the form of Sodium arsenite (NaAsO2, M.W. =129.91) were added to the modified Hoagland nutrient solution@ 2.5, 5.0, 7.5, 10.0, 12.5,15.0 mg L-1concentration. After 20 days of treatment, rice seedlings under arsenate/arsenite treatments were analyzed for change in total chlorophyll content and antioxidative enzymes activity such as superoxide dismutase (SOD: EC 1.15.1.1), catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC1.11.1.11) and peroxidase (POD: EC 1.11.1.7). Results: A noticeable decrease in total chlorophyll content was observed with arsenic treatments as compared to control. Various antioxidative enzymes showed significant variable response upon exposure to As(III) and As(V). SOD activities increased at low arsenic exposure up to 7.5 mg L-1 but decreased with further increase in arsenic treatments.  APX and POD activities were increased with increase in arsenic concentrations while CAT activity displayed decreasing trend. Conclusion: The results are suggestive of differential metabolism of As(III) and As(V) in rice and could exert harmfulness in the early development stage of rice at inappropriate concentrations.

Antioxidative enzymes arsenic stress catalase chlorophyll peroxidase rice super oxide dismutase.

References (23)

  1. 1 COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS [DOI]
  2. 2 Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts [DOI]
  3. 3 A SPECTROPHOTOMETRIC METHOD FOR MEASURING THE BREAKDOWN OF HYDROGEN PEROXIDE BY CATALASE [DOI]
  4. 4 Superoxide Dismutases [DOI]
  5. 5 Transporters of arsenite in rice and their role in arsenic accumulation in rice grain [DOI]
  6. 6 The influence of chemical form and concentration of arsenic on rice growth and tissue arsenic concentration [DOI]
  7. 7 Rice is more efficient in arsenite uptake and translocation than wheat and barley [DOI]
  8. 8 Effect of arsenic on photosynthesis, growth and yield of five widely cultivated rice (Oryza sativa L.) varieties in Bangladesh [DOI]
  9. 9 Relative toxicity of arsenite and arsenate on germination and early seedling growth of rice (Oryza sativa L.) [DOI]
  10. 10 Arsenic induced oxidative stress in plants [DOI]
  11. 11 Arsenic removal from drinking water by flocculation and microfiltration [DOI]
  12. 12 An update on nitric oxide and its benign role in plant responses under metal stress [DOI]
  13. 13 Effect of compost addition on arsenic uptake, morphological and physiological attributes of maize plants grown in contrasting soils [DOI]
  14. 14 Biological responses of duckweed (Lemna minor L.) exposed to the inorganic arsenic species As(III) and As(V): effects of concentration and duration of exposure [DOI]
  15. 15 Arsenic and other heavy metal accumulation in plants and algae growing naturally in contaminated area of West Bengal, India [DOI]
  16. 16 Effect of selenium induced seed priming on arsenic accumulation in rice plant and subsequent transmission in human food chain [DOI]
  17. 17 Impact of silicon on Indian mustard (Brassica juncea L.) root traits by regulating growth parameters, cellular antioxidants and stress modulators under arsenic stress [DOI]
  18. 18 Differential tolerance of canola to arsenic when grown hydroponically or in soil [DOI]
  19. 19 Assessment of Arsenic in the Water-Soil-Plant Systems in Gangetic Floodplains of Bangladesh [DOI]
  20. 20 Rice seed priming with Se : A novel approach to mitigate As induced adverse consequences on growth, yield and As load in brown rice [DOI]
  21. 21 Agroecotoxicological Aspect of Arsenic (As) and Cadmium (Cd) on Field Crops and its Mitigation: Current Status and Future Prospect [DOI]
  22. 22 Recent Advances in Biotechnology and Genomic Approaches for Abiotic Stress Tolerance in Crop Plants [DOI]
  23. 23 Effect of salinity on rhizosphere acidification and antioxidant activity of two acacia species [DOI]

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